Forster G E, Cookey I, Munday P E, Richman P I, Jha R, Coleman D, Thomas B J, Hawkins D A, Evans R T, Taylor-Robinson D
J Clin Pathol. 1985 Apr;38(4):399-402. doi: 10.1136/jcp.38.4.399.
Forty five (37%) of 121 female contacts of men with non-gonococcal urethritis or gonorrhoea were chlamydia positive, as judged by isolation or by detecting elementary bodies in smears with a fluorescein labelled chlamydial monoclonal antibody. Only six (13%) of these, however, had Papanicolaou stained smears in which there were inclusion like changes suggestive of chlamydial infection. Furthermore, of 15 patients who had such cytological changes, chlamydiae were detected in only six and the abnormalities were found also in Papanicolaou stained smears from 10 (13%) of the 76 chlamydia negative patients. Modifying the Papanicolaou stained smears by including endocervical material did not increase sensitivity. In addition, destaining and restaining them with the monoclonal antibody was time consuming and the results were unreliable. The staining of cervical smears with Papanicolaou reagent is a technique of low sensitivity and specificity for diagnosing or screening for chlamydial cervical infection and cannot be recommended.
121名非淋菌性尿道炎或淋病男性患者的女性性伴侣中，45名（37%）衣原体检测呈阳性，检测方法为分离培养或用荧光素标记的衣原体单克隆抗体涂片检测原体。然而，其中只有6名（13%）的巴氏涂片显示有类似包涵体的变化，提示衣原体感染。此外，15名有此类细胞学变化的患者中，仅6名检测到衣原体，且在76名衣原体阴性患者的巴氏涂片中，有10名（13%）也发现了异常。通过增加宫颈管内取材来改良巴氏涂片并不能提高敏感性。此外，用单克隆抗体对涂片进行脱色和复染既耗时，结果也不可靠。用巴氏试剂对宫颈涂片进行染色，在诊断或筛查衣原体宫颈感染方面，是一种敏感性和特异性较低的技术，不推荐使用。
