Lima Cássio A, Goulart Viviane P, Côrrea Luciana, Pereira Thiago M, Zezell Denise M
Center for Lasers and Applications, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo SP 05508-000, Brazil.
School of Dentistry, Universidade de São Paulo, São Paulo SP 05508-000, Brazil.
Int J Mol Sci. 2015 Mar 24;16(4):6621-30. doi: 10.3390/ijms16046621.
Nonmelanoma skin cancers represent 95% of cutaneous neoplasms. Among them, squamous cell carcinoma (SCC) is the more aggressive form and shows a pattern of possible metastatic profile. In this work, we used Fourier transform infrared spectroscopy (FTIR) spectroscopy to assess the biochemical changes in normal skin caused by squamous cell carcinoma induced by multi-stage chemical carcinogenesis in mice. Changes in the absorption intensities and shifts were observed in the vibrational modes associated to proteins, indicating changes in secondary conformation in the neoplastic tissue. Hierarchical cluster analysis was performed to evaluate the potential of the technique to differentiate the spectra of neoplastic and normal skin tissue, so that the accuracy obtained for this classification was 86.4%. In this sense, attenuated total reflection (ATR)-FTIR spectroscopy provides a useful tool to complement histopathological analysis in the clinical routine for the diagnosis of cutaneous squamous cell carcinoma.
非黑色素瘤皮肤癌占皮肤肿瘤的95%。其中，鳞状细胞癌（SCC）是更具侵袭性的类型，且显示出可能的转移特征模式。在这项工作中，我们使用傅里叶变换红外光谱（FTIR）来评估小鼠多阶段化学致癌诱导的鳞状细胞癌对正常皮肤造成的生化变化。在与蛋白质相关的振动模式中观察到吸收强度和位移的变化，表明肿瘤组织的二级构象发生了变化。进行了层次聚类分析，以评估该技术区分肿瘤和正常皮肤组织光谱的潜力，该分类获得的准确率为86.4%。从这个意义上说，衰减全反射（ATR）-FTIR光谱为临床常规诊断皮肤鳞状细胞癌的组织病理学分析提供了一种有用的补充工具。
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