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[大鼠交感神经细胞出生后个体发育过程中核染色质蛋白质成分的细胞学分析。I. 氨银染色可检测到的组蛋白的年龄相关变化及大鼠交感神经细胞染色质的基质活性]

[Cytological analysis of the protein component of the nuclear chromatin in rat sympathetic neurocytes in postnatal ontogeny. I. The age-related changes in the histones detectable by ammoniacal silver and the matrix activity of the chromatin in rat sympathetic neurocytes].

作者信息

Grigor'eva A V, Iarygin V N

出版信息

Tsitologiia. 1985 Feb;27(2):186-90.

PMID:2581341
Abstract

The nuclear histones of the rat superior cervical ganglion neurons were stained at different stages of postnatal ontogenesis with ammoniacal silver (AS). Considerable changes in neuronal staining were revealed during the terminal cytodifferentiation, whereas no changes were found during mature age and in a more advanced age. A combined method was proposed that involved simultaneous AS-staining and radioautographical evaluation of transcription in the same cell. No correlation was seen between the nuclear AS-staining and the number of active transcription units.

摘要

用氨性银(AS)对大鼠颈上神经节神经元的核组蛋白在出生后个体发育的不同阶段进行染色。在终末细胞分化过程中发现神经元染色有显著变化,而在成熟和更老龄阶段未发现变化。提出了一种联合方法,该方法涉及在同一细胞中同时进行AS染色和转录的放射自显影评估。未观察到核AS染色与活跃转录单位数量之间的相关性。

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