Department of Plant Sciences, University of California, Davis Davis, CA, USA ; Departamento de Genética e Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas Campinas, Brazil ; Centro de Pesquisa e Desenvolvimento em Recursos Genéticos Vegetais, Instituto Agronômico de Campinas-IAC Campinas, Brazil.
Departamento de Genética e Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas Campinas, Brazil.
Front Plant Sci. 2015 Mar 12;6:152. doi: 10.3389/fpls.2015.00152. eCollection 2015.
Genetic resistance of common bean (Phaseolus vulgaris L.) against angular leaf spot (ALS), caused by the fungus Pseudocercospora griseola, is conferred by quantitative trait loci (QTL). In this study, we determined the gene content of the major QTL ALS10.1 located at the end of chromosome Pv10, and identified those that are responsive to ALS infection in resistant (CAL 143) and susceptible (IAC-UNA) genotypes. Based on the current version of the common bean reference genome, the ALS10.1 core region contains 323 genes. Gene Ontology (GO) analysis of these coding sequences revealed the presence of genes involved in signal perception and transduction, programmed cell death (PCD), and defense responses. Two putative R gene clusters were found at ALS10.1 containing evolutionary related coding sequences. Among them, the Phvul.010G025700 was consistently up-regulated in the infected IAC-UNA suggesting its contribution to plant susceptibility to the fungus. We identified six other genes that were regulated during common bean response to P. griseola; three of them might be negative regulators of immunity as they showed opposite expression patterns during resistant and susceptible reactions at the initial phase of fungal infection. Taken together, these findings suggest that common bean reaction to P. griseola involves transcriptional modulation of defense genes in the ALS10.1 locus, contributing to resistance or susceptibility depending on the plant-pathogen interaction.
普通菜豆（Phaseolus vulgaris L.）对由真菌 Pseudocercospora griseola 引起的角斑病（ALS）的遗传抗性由数量性状位点（QTL）赋予。在这项研究中，我们确定了位于染色体 Pv10 末端的主要 ALS10.1 QTL 的基因含量，并鉴定了在抗性（CAL 143）和敏感（IAC-UNA）基因型中对 ALS 感染有反应的基因。基于普通菜豆参考基因组的当前版本，ALS10.1 核心区域包含 323 个基因。这些编码序列的基因本体论（GO）分析显示存在参与信号感知和转导、细胞程序性死亡（PCD）和防御反应的基因。在 ALS10.1 中发现了两个假定的 R 基因簇，其中包含进化相关的编码序列。其中，Phvul.010G025700 在感染的 IAC-UNA 中持续上调，表明其对植物易感性的贡献。我们鉴定了另外六个在普通菜豆对 P. griseola 反应过程中受到调节的基因；其中三个可能是免疫的负调节剂，因为它们在真菌感染初期的抗性和敏感反应中表现出相反的表达模式。总之，这些发现表明，普通菜豆对 P. griseola 的反应涉及 ALS10.1 基因座中防御基因的转录调节，根据植物-病原体相互作用，有助于抗性或敏感性。
