Chandel Kshitij, Parikh Rasesh Y, Mendki Murlidhar J, Shouche Yogesh S, Veer Vijay
Vector Management Division, Defence Research & Development Establishment, Gwalior, India.
J Vector Borne Dis. 2015 Mar;52(1):52-7.
BACKGROUND & OBJECTIVES: Mosquito gut is a rich source of microorganisms. These microorganisms exhibit close association and contribute various physiological processes taking place in mosquito gut. The present study is aimed to characterize two bacterial isolates M19 and GB11 recovered from the gut of Culex quinquefasciatus mosquito collected from Bhuj and Jamnagar districts of Gujarat, India.
Both the strains were characterized using polyphasic approach including, phenotypic characterization, whole cell protein profiling and sequencing of 16S rRNA gene and groESL region.
Sequences of 16S rRNA gene of M19 and GB11 were 99% similar to Vagococcus carniphilus and Vagococcus fluvialis. But phenotypic profile, whole cell protein profile and sequence of groESL region of both isolates were found to be similar to V. fluvialis.
Based on phenotypic, genotypic and protein profiling, both the strains were identified as V. fluvialis. So far this species was known from domestic animals and human sources only. This is the first report of V. fluvialis inhabiting midgut of Cx. quinquefasciatus mosquito collected from Arabian sea coastal of India.
蚊虫肠道是微生物的丰富来源。这些微生物表现出密切关联，并对蚊虫肠道内发生的各种生理过程发挥作用。本研究旨在对从印度古吉拉特邦布杰和贾姆讷格尔地区采集的致倦库蚊肠道中分离出的两株细菌M19和GB11进行特性分析。
采用多相方法对这两株菌株进行特性分析，包括表型特征分析、全细胞蛋白图谱分析以及16S rRNA基因和groESL区域测序。
M19和GB11的16S rRNA基因序列与嗜肉漫游球菌和河流漫游球菌的相似度为99%。但两株分离菌的表型特征、全细胞蛋白图谱以及groESL区域序列均与河流漫游球菌相似。
基于表型、基因型和蛋白图谱分析，这两株菌株均被鉴定为河流漫游球菌。此前该物种仅见于家畜和人类来源。这是关于河流漫游球菌栖息于从印度阿拉伯海沿岸采集的致倦库蚊中肠的首次报道。
