Yadav Kamlesh K, Bora Ajitabh, Datta Sibnarayan, Chandel Kshitij, Gogoi Hemant K, Prasad G B K S, Veer Vijay
Biotechnology Division, Defence Research Laboratory, DRDO, Post Bag 2, Tezpur, Assam, India.
Molecular Virology Laboratory, Defence Research Laboratory, DRDO, Post Bag 2, Tezpur, Assam, India.
Parasit Vectors. 2015 Dec 18;8:641. doi: 10.1186/s13071-015-1252-0.
Microbiota inhabiting midguts of mosquitoes play a key role in the host - parasite interaction and enhance vectorial capacity of viral diseases like dengue and chikungunya fevers. Mosquito midgut is considered to be an important site for host-pathogen interaction and pathogen survival is thought to be an outcome of this interaction. In the present study we examined the bacterial community in the midgut of Aedes mosquitoes in Arunanchal Pradesh, India, a subtropical zone where dengue fever is reported to be emerging.
Larvae and pupa of Aedes mosquitoes were collected from a biodiversity hotspot, Bhalukpong, Arunachal Pradesh, India. 16S rRNA gene sequences were used for identification of isolated bacterial population from each species of mosquitoes. We used various diversity indices to assess the diversity and richness of the bacterial isolates in both mosquito species.
On the basis of 16S rRNA gene sequence analysis a total of 24 bacterial species from 13 genera were identified belonging to 10 families of four major phyla. Phylum Proteobacteria was dominant followed by Firmicutes, Bacteroidetes and Actinobacteria. The midgut bacteria belonging to the phylum Proteobacteria and Firmicutes were isolated from both Ae. albopictus and Ae. aegypti, whereas, bacteria belonging to phylum Bacteroidetes and Actinobacteria were isolated only from Ae. albopictus and Ae. aegypti respectively. Enterobacter cloacae was the dominant bacterial species in both Ae. albopictus (33.65%) and Ae. aegypti (56.45%). Bacillus aryabhattai (22.78%) was the second most common bacterial species in Ae. albopictus whereas, in Ae. aegypti the second most common bacterial species was Stenotrophomonas maltophilia (7.44%).
The family Enterobacteriaceae of phylum Proteobacteria was dominant in both species of Aedes mosquitoes. To the best of our knowledge, this is the first attempt to study midgut microbiota from a biodiversity hotspot in Northeastern India. Some bacterial genera Enterobacter and Acinetobacter isolated in this study are known to play important roles in parasite-vector interaction. Information on midgut microflora may lead towards the development of novel, safe, and effective strategies to manipulate the vectorial capacity of mosquitoes.
栖息于蚊子中肠的微生物群在宿主 - 寄生虫相互作用中起关键作用，并增强登革热和基孔肯雅热等病毒性疾病的传播能力。蚊子中肠被认为是宿主 - 病原体相互作用的重要场所，病原体的存活被认为是这种相互作用的结果。在本研究中，我们调查了印度阿鲁纳恰尔邦（一个亚热带地区，据报道登革热正在出现）埃及伊蚊中肠的细菌群落。
从印度阿鲁纳恰尔邦巴卢孔的一个生物多样性热点地区收集埃及伊蚊的幼虫和蛹。16S rRNA基因序列用于鉴定从每种蚊子分离出的细菌种群。我们使用各种多样性指数来评估两种蚊子中细菌分离株的多样性和丰富度。
基于16S rRNA基因序列分析，共鉴定出13个属的24种细菌，属于四个主要门的10个科。变形菌门占主导地位，其次是厚壁菌门、拟杆菌门和放线菌门。属于变形菌门和厚壁菌门的中肠细菌从白纹伊蚊和埃及伊蚊中均有分离，而属于拟杆菌门和放线菌门的细菌分别仅从白纹伊蚊和埃及伊蚊中分离得到。阴沟肠杆菌是白纹伊蚊（33.65%）和埃及伊蚊（56.45%）中的优势细菌物种。芽孢杆菌（22.78%）是白纹伊蚊中第二常见的细菌物种，而在埃及伊蚊中第二常见的细菌物种是嗜麦芽窄食单胞菌（7.44%）。
变形菌门肠杆菌科在两种埃及伊蚊中均占主导地位。据我们所知，这是首次尝试研究印度东北部生物多样性热点地区的中肠微生物群。本研究中分离出的一些细菌属，如肠杆菌属和不动杆菌属，已知在寄生虫 - 媒介相互作用中起重要作用。关于中肠微生物区系的信息可能会引领开发新的、安全且有效的策略来控制蚊子的传播能力。
