Berman R, Gusterson B, Steel G G
Br J Cancer. 1985 May;51(5):653-8. doi: 10.1038/bjc.1985.98.
Small cell bronchial carcinoma (SCC) xenografts with differing sensitivity to cyclophosphamide (CY) were investigated using a variety of techniques. Two xenografts (HX78 and HX88) were relatively sensitive to CY, one xenograft (HX72) was inherently resistant to CY and a fourth xenograft (HX78Cy) was a CY induced resistant subline of HX78 and was unstable when maintained without CY exposure. Conventional light microscopy, cytology and electron microscopy examination of the xenografts revealed appearances consistent with SCC. An antikeratin antibody demonstrated the presence of keratin in the inherent and the induced resistant xenografts (and in the unstable revertant) but not in the two sensitive xenografts; the presence of keratin suggested squamous differentiation. Monolayer culture morphology of the sensitive HX78 and the unstable revertant was anchorage independent with the cells forming floating aggregates; the induced resistant subline of this xenograft (HX78Cy) showed, by contrast, flattened, angular adherent cells. Beta HCG production was detected in the monolayer culture supernatant of sensitive HX78 cells; the level of production of beta HCG was increased in the induced resistant HX78Cy cells. Karyotype and flow cytometry studies were also performed. The morphological responses of small cell lung cancer to treatment are discussed.
利用多种技术对具有不同环磷酰胺（CY）敏感性的小细胞支气管癌（SCC）异种移植瘤进行了研究。两种异种移植瘤（HX78和HX88）对CY相对敏感，一种异种移植瘤（HX72）对CY固有耐药，第四种异种移植瘤（HX78Cy）是HX78的CY诱导耐药亚系，在无CY暴露的情况下维持时不稳定。对异种移植瘤进行常规光学显微镜、细胞学和电子显微镜检查，发现其表现与SCC一致。一种抗角蛋白抗体显示，固有耐药和诱导耐药的异种移植瘤（以及不稳定的回复株）中存在角蛋白，而两种敏感的异种移植瘤中不存在；角蛋白的存在提示鳞状分化。敏感的HX78和不稳定回复株的单层培养形态不依赖贴壁，细胞形成漂浮聚集体；相比之下，该异种移植瘤的诱导耐药亚系（HX78Cy）显示扁平、有角的贴壁细胞。在敏感的HX78细胞单层培养上清液中检测到β-HCG的产生；诱导耐药的HX78Cy细胞中β-HCG的产生水平升高。还进行了核型和流式细胞术研究。讨论了小细胞肺癌对治疗的形态学反应。
