Berman R, Steel G G
Br J Cancer. 1984 Apr;49(4):431-6. doi: 10.1038/bjc.1984.69.
Inherent and induced resistance was investigated in human small-cell lung cancer xenografts. Specimens from three patients were established in immune suppressed mice; the sensitivity of the xenografts to cyclophosphamide, MeCCNU and melphalan was determined using the growth delay end-point. Clinical chemosensitivity data were available in two cases and inherent differences in sensitivity were noted both in the xenografts and clinically. Radioactively labelled melphalan uptake studies were performed with these two xenografts. A number of different strategies to induce resistance were explored. Only one method proved to be successful and in only one of the xenografts; this was with cyclophosphamide. The induced resistant line was characterised in terms of the time course of its production, the degree of induced resistance, the growth rate, the cross-resistance pattern and stability of the phenotype; the possibility of altered antigenicity was also examined.
在人小细胞肺癌异种移植模型中研究了固有抗性和诱导抗性。取自三名患者的标本被植入免疫抑制小鼠体内；使用生长延迟终点来确定异种移植对环磷酰胺、甲基环己亚硝脲和马法兰的敏感性。两例患者有临床化疗敏感性数据，异种移植和临床中均发现了敏感性的固有差异。对这两种异种移植进行了放射性标记马法兰摄取研究。探索了多种诱导抗性的不同策略。仅有一种方法被证明是成功的，且仅在其中一种异种移植中成功；这种方法是使用环磷酰胺。对诱导抗性细胞系在其产生的时间进程、诱导抗性程度、生长速率、交叉抗性模式和表型稳定性方面进行了表征；还研究了抗原性改变的可能性。
