Zakrzewska Karolina Ewa, Samluk Anna, Wierzbicki Mateusz, Jaworski Sławomir, Kutwin Marta, Sawosz Ewa, Chwalibog André, Pijanowska Dorota Genowefa, Pluta Krzysztof Dariusz
Department of Hybrid Microbiosystem Engineering, Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland.
Division of Nanobiotechnology, Faculty of Animal Science, Warsaw University of Life Sciences, Warsaw, Poland.
PLoS One. 2015 Mar 27;10(3):e0122579. doi: 10.1371/journal.pone.0122579. eCollection 2015.
Nanoparticles have attracted a great deal of attention as carriers for drug delivery to cancer cells. However, reports on their potential cytotoxicity raise questions of their safety and this matter needs attentive consideration. In this paper, for the first time, the cytotoxic effects of two carbon based nanoparticles, diamond and graphite, on glioblastoma and hepatoma cells were compared. First, we confirmed previous results that diamond nanoparticles are practically nontoxic. Second, graphite nanoparticles exhibited a negative impact on glioblastoma, but not on hepatoma cells. The studied carbon nanoparticles could be a potentially useful tool for therapeutics delivery to the brain tissue with minimal side effects on the hepatocytes. Furthermore, we showed the influence of the nanoparticles on the stable, fluorescently labeled tumor cell lines and concluded that the labeled cells are suitable for drug cytotoxicity tests.
纳米颗粒作为向癌细胞递送药物的载体已引起了广泛关注。然而，关于其潜在细胞毒性的报道引发了对其安全性的质疑，此事需要认真考虑。在本文中，首次比较了两种碳基纳米颗粒——金刚石和石墨——对胶质母细胞瘤细胞和肝癌细胞的细胞毒性作用。首先，我们证实了先前的结果，即金刚石纳米颗粒实际上是无毒的。其次，石墨纳米颗粒对胶质母细胞瘤有负面影响，但对肝癌细胞没有影响。所研究的碳纳米颗粒可能是一种潜在有用的工具，可用于向脑组织递送治疗药物，同时对肝细胞产生最小的副作用。此外，我们展示了纳米颗粒对稳定的、荧光标记的肿瘤细胞系的影响，并得出结论，标记的细胞适用于药物细胞毒性测试。
