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利用水力空化同时处理湿微藻(细胞破碎和脂质提取),以提高脂质产率。

Simultaneous treatment (cell disruption and lipid extraction) of wet microalgae using hydrodynamic cavitation for enhancing the lipid yield.

机构信息

Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.

Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.

出版信息

Bioresour Technol. 2015 Jun;186:246-251. doi: 10.1016/j.biortech.2015.03.045. Epub 2015 Mar 13.

DOI:10.1016/j.biortech.2015.03.045
PMID:25817036
Abstract

Simultaneous treatment (combining with cell disruption and lipid extraction) using hydrodynamic cavitation (HC) was applied to Nannochloropsis salina to demonstrate a simple and integrated way to produce oil from wet microalgae. A high lipid yield from the HC (25.9-99.0%) was observed compared with autoclave (16.2-66.5%) and ultrasonication (5.4-26.9%) in terms of the specific energy input (500-10,000 kJ/kg). The optimal conditions for the simultaneous treatment were established using a statistical approach. The efficiency of the simultaneous method was also demonstrated by comparing each separate treatment. The maximum lipid yield (predicted: 45.9% and experimental: 45.5%) was obtained using 0.89% sulfuric acid with a cavitation number of 1.17 for a reaction time of 25.05 min via response surface methodology. Considering its comparable extractability, energy-efficiency, and potential for scale-up, HC may be a promising method to achieve industrial-scale microalgae operation.

摘要

采用水力空化(HC)同时进行处理(结合细胞破碎和脂质提取),应用于小球藻(Nannochloropsis salina),展示了一种从湿微藻中生产油脂的简单而综合的方法。与高压釜(16.2-66.5%)和超声处理(5.4-26.9%)相比,就特定能量输入(500-10000 kJ/kg)而言,HC 的油脂得率较高(25.9-99.0%)。通过统计方法确定了同时处理的最佳条件,并通过比较每种单独的处理方法证明了同时处理方法的效率。通过响应面法,使用 0.89%硫酸和空化数为 1.17,反应时间为 25.05 分钟,获得了最大油脂产率(预测值:45.9%,实验值:45.5%)。考虑到其可比拟的提取效率、能量效率和扩大规模的潜力,HC 可能是实现工业规模微藻操作的一种有前途的方法。

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