Servaes K, Maesen M, Prandi B, Sforza S, Elst K
†Unit Separation and Conversion Technology, Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium.
‡Department of Food Science, University of Parma, Viale delle Scienze 59/A University Campus, 43124 Parma, Italy.
J Agric Food Chem. 2015 Apr 22;63(15):3931-41. doi: 10.1021/acs.jafc.5b00241. Epub 2015 Apr 8.
Lipid compositions obtained from microalgae species are affected by both the cultivation conditions and the extraction method used. In this study, the extraction of lipids from Nannochloropsis oculata using traditional and modern extraction technologies with several solvents has been compared. Because important polyunsaturated fatty acids are bound to polar lipids, these polar lipids were the main focus of this study. The dominant compounds in the glycolipid fractions were monogalactosyldiglycerides and digalactosyldiglycerides bearing fatty acid chains containing at least one site of unsaturation. Phosphatidylcholine and trimethylhomoserines were detected in the phospholipid fractions. The fatty acid profile comprised large fractions of C16:0, C16:1, C20:5, and C18:3. Extraction of specific compounds was determined by extraction efficiency as well as differences in the selectivity of the method used. The composition derived from a glycolipid fraction was observed to be affected by the method used to a greater extent than the phospholipid fraction.
从微藻物种中获得的脂质成分受培养条件和所用提取方法的影响。在本研究中，比较了使用几种溶剂通过传统和现代提取技术从眼点拟微球藻中提取脂质的情况。由于重要的多不饱和脂肪酸与极性脂质结合，这些极性脂质是本研究的主要重点。糖脂组分中的主要化合物是带有含有至少一个不饱和位点的脂肪酸链的单半乳糖基二甘油酯和二半乳糖基二甘油酯。在磷脂组分中检测到磷脂酰胆碱和三甲基高丝氨酸。脂肪酸谱包含大量的C16:0、C16:1、C20:5和C18:3。特定化合物的提取取决于提取效率以及所用方法选择性的差异。观察到糖脂组分衍生的成分比磷脂组分受所用方法的影响更大。
