Lotz M, Tsoukas C D, Fong S, Carson D A, Vaughan J H
Eur J Immunol. 1985 May;15(5):520-5. doi: 10.1002/eji.1830150518.
Interferons (IFN) are antiviral proteins that may be important in mediating cellular defenses against Epstein-Barr virus (EBV) infection. However, the means by which IFN-alpha, -beta and -gamma modify EBV infectivity are not clear. We have evaluated the effects of purified recombinant preparations of all three classes of IFN on EBV-induced B lymphocyte proliferation and Ig secretion. When added early after EBV infection, all three recombinant IFN reduced B cell outgrowth and Ig secretion. IFN-gamma exerted a 7-10-fold more potent antiviral effect than IFN-alpha or -beta. All three types of IFN act directly on B cells. Monocytes and natural killer cells are not necessary for the anti-EBV activity. Of the three recombinant IFN, only IFN-gamma reduced EBV-induced proliferation and Ig secretion when added 3-4 days after virus infection; IFN-alpha/beta were only effective up to 24 h. B lymphoblastoid lines already transformed by EBV are insensitive to the anti-proliferative actions of all three types of IFN. On the basis of these findings, we propose three phases of regulation during EBV infection. In the early phase, EBV-infected cells can be regulated by all IFN. Subsequently, there is an intermediate period where only IFN-gamma is capable of directly affecting EBV-induced B cell responses. In the third phase, B lymphocytes become insensitive to direct actions of all IFN and are now subject to regulation only by cytotoxic cells.
干扰素（IFN）是抗病毒蛋白，在介导细胞抵御爱泼斯坦-巴尔病毒（EBV）感染方面可能很重要。然而，α、β和γ干扰素改变EBV感染性的方式尚不清楚。我们评估了所有三类干扰素的纯化重组制剂对EBV诱导的B淋巴细胞增殖和免疫球蛋白分泌的影响。在EBV感染后早期添加时，所有三种重组干扰素均降低了B细胞的生长和免疫球蛋白分泌。γ干扰素的抗病毒作用比α或β干扰素强7至10倍。所有三种类型的干扰素均直接作用于B细胞。单核细胞和自然杀伤细胞对于抗EBV活性并非必需。在三种重组干扰素中，只有γ干扰素在病毒感染后3至4天添加时可降低EBV诱导的增殖和免疫球蛋白分泌；α/β干扰素仅在24小时内有效。已被EBV转化的B淋巴母细胞系对所有三种类型干扰素的抗增殖作用均不敏感。基于这些发现，我们提出EBV感染期间的三个调节阶段。在早期阶段，EBV感染的细胞可受所有干扰素调节。随后，存在一个中间期，只有γ干扰素能够直接影响EBV诱导的B细胞反应。在第三阶段，B淋巴细胞对所有干扰素的直接作用变得不敏感，现在仅受细胞毒性细胞调节。
