Büki K G, Seppä H
FEBS Lett. 1985 May 20;184(2):254-8. doi: 10.1016/0014-5793(85)80617-7.
The formation of new blood vessels occurs by sprouting from previously existing microvasculature. The process involved directed migration of the vascular endothelial cells towards chemical signals released from the target tissue. We have used the Boyden chemotaxis chamber method to identify chemotactic signals for fetal bovine vascular endothelial cells. Human placenta organ cultures produce a high-Mr chemoattractant for the endothelial cells from which a low-Mr factor can be liberated with trichloroacetic acid treatment and ethanol extraction. This activity was isolated from extracts of human placenta using Sephadex LH-20, Amberlite XAD-2, and silica gel thin-layer chromatography. The Mr of the factor is less than 400, it is lipophilic and resistant to proteolytic enzymes. The factor induces chemotactic migration of both aortic endothelial cells and capillary endothelial cells from the retina, but has no effect on fibroblasts or leukocytes suggesting a specific function of the compound for the vascular endothelial cells.
新血管的形成是通过从先前存在的微脉管系统发芽而发生的。该过程涉及血管内皮细胞朝着靶组织释放的化学信号进行定向迁移。我们使用博伊登趋化室方法来鉴定胎牛血管内皮细胞的趋化信号。人胎盘器官培养物产生一种高分子量的趋化剂，用于内皮细胞，用三氯乙酸处理和乙醇提取可从中释放出一种低分子量因子。使用葡聚糖凝胶LH - 20、Amberlite XAD - 2和硅胶薄层色谱从人胎盘提取物中分离出这种活性物质。该因子的分子量小于400，具有亲脂性且对蛋白水解酶有抗性。该因子可诱导主动脉内皮细胞和视网膜毛细血管内皮细胞的趋化迁移，但对成纤维细胞或白细胞没有影响，表明该化合物对血管内皮细胞具有特定功能。
