内皮细胞在体外对血小板衍生生长因子样趋化因子的极化分泌
Polarized secretion of a platelet-derived growth factor-like chemotactic factor by endothelial cells in vitro.
作者信息
Zerwes H G, Risau W
机构信息
Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Federal Republic of Germany.
出版信息
J Cell Biol. 1987 Nov;105(5):2037-41. doi: 10.1083/jcb.105.5.2037.
Abstract
Cultured bovine aortic endothelial cells secrete a potent migration-stimulating factor for vascular smooth muscle cells (SMCs) and adventitial fibroblasts. Vascular pericytes are 20-fold less responsive, and endothelial cells themselves do not respond at all. Checkerboard analysis of SMC migration in a micro-chemotaxis chamber assay shows that the factor is chemotactic. Chemotactic activity for SMCs and adventitial fibroblasts is specifically inhibited by antibodies against platelet-derived growth factor. Endothelial cells cultured on nitrocellulose filters secrete the platelet-derived growth factor-like factor almost exclusively into the basal compartment. We suggest that this factor plays an important role in the recruitment of vascular wall cells during the morphogenesis of blood vessels and pathological conditions, such as atherosclerosis.
摘要
培养的牛主动脉内皮细胞分泌一种对血管平滑肌细胞（SMC）和外膜成纤维细胞有强大迁移刺激作用的因子。血管周细胞的反应性低20倍，而内皮细胞本身则完全没有反应。在微趋化室试验中对SMC迁移进行的棋盘分析表明，该因子具有趋化作用。针对血小板衍生生长因子的抗体可特异性抑制SMC和外膜成纤维细胞的趋化活性。在硝酸纤维素滤膜上培养的内皮细胞几乎只将血小板衍生生长因子样因子分泌到基底室。我们认为，该因子在血管形态发生和诸如动脉粥样硬化等病理状况期间血管壁细胞的募集过程中发挥重要作用。
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