Yang Gi-Hoon, Kim Minseong, Kim GeunHyung
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
J Colloid Interface Sci. 2015 Jul 15;450:159-167. doi: 10.1016/j.jcis.2015.02.070. Epub 2015 Mar 18.
Hybrid biomedical structures have been used widely in various tissue-regenerating materials because they effectively induce exceptional physical and cellular responses. In this study, a new hybrid process was used to design a three-dimensional (3D) biomedical hybrid scaffold with a controlled pore-structure and high mechanical strength. A melt-dispensing method was used to obtain mechanical properties and electrohydrodynamic direct-jet (EHD-DJ) printing was used to provide microsized fibrous structures for the scaffold. Furthermore, the poly(ε-caprolactone) (PCL) hybrid scaffolds were coated biomimetically with type-I collagen to increase bioactive interactions between cells and scaffolds. The fabricated scaffolds showed similar mechanical properties to the two control scaffolds; however, the results of culturing osteoblast-like (MG63) cells showed significant increases in in vitro cellular activities (cell viability>twofold and calcium deposition>sevenfold). Based on these results, we propose a newly designed hybrid scaffold that can support significant in vitro cellular activities at the interface between cells and the 3D micro-pore structure for soft and hard tissue regeneration.
混合生物医学结构已广泛应用于各种组织再生材料中，因为它们能有效引发特殊的物理和细胞反应。在本研究中，采用了一种新的混合工艺来设计具有可控孔结构和高机械强度的三维（3D）生物医学混合支架。使用熔体挤出法来获得机械性能，并采用电液动力直接喷射（EHD-DJ）打印为支架提供微尺寸纤维结构。此外，聚（ε-己内酯）（PCL）混合支架通过仿生方式涂覆I型胶原蛋白，以增强细胞与支架之间的生物活性相互作用。所制备的支架显示出与两种对照支架相似的机械性能；然而，培养成骨样（MG63）细胞的结果表明，体外细胞活性显著增加（细胞活力增加两倍以上，钙沉积增加七倍以上）。基于这些结果，我们提出了一种新设计的混合支架，它能够在细胞与3D微孔结构的界面处支持显著的体外细胞活性，用于软组织和硬组织再生。
