Lavayre J, Leng M
C R Acad Hebd Seances Acad Sci D. 1976 Dec 20;283(16):1819-22.
Antibodies to ApA elicited in Rabbits by immunization with bovine serum albumin-ApA conjugate have been purified by affinity chromatography. These purified antibodies react with bovine serum albumin-AMP, 8 bromo AMP, ApA conjugates as shown by gel diffusion. The association constants for the binding of antibodies and several haptens were deduced from dialysis equilibrium. The number of bound hapten molecules per antibody binding site depends upon the nature of the hapten.
通过用牛血清白蛋白-ApA 偶联物免疫兔子产生的抗 ApA 抗体已通过亲和层析纯化。如凝胶扩散所示,这些纯化的抗体与牛血清白蛋白-AMP、8-溴 AMP、ApA 偶联物发生反应。抗体与几种半抗原结合的缔合常数由透析平衡推导得出。每个抗体结合位点结合的半抗原分子数量取决于半抗原的性质。