Holm Dorte K, Moessner Belinda K, Engle Ronald E, Zaaijer Hans L, Georgsen Jørgen, Purcell Robert H, Christensen Peer B
Department of Clinical Immunology, Odense University Hospital, Odense, Denmark.
Department of Infectious Diseases, Odense University Hospital, Odense, Denmark.
Transfusion. 2015 Jul;55(7):1662-7. doi: 10.1111/trf.13028. Epub 2015 Mar 27.
The increasing incidence of reported hepatitis E cases in Europe has focused attention on hepatitis E virus (HEV) and the risk of transfusion-transmitted hepatitis E. The aim of this study was to investigate the prevalence of antibodies to HEV (anti-HEV) among Danish blood donors in 2013 and to compare it to previous studies in Denmark. In addition we wanted to compare the relative reactivity of two different assays.
Samples from 504 blood donors were collected and analyzed for anti-HEV with an in-house assay developed at the National Institutes of Health (NIH). In addition the samples were analyzed with the Wantai anti-HEV assay. Demographic information and possible HEV exposure was collected by self-administered questionnaire.
Using the NIH assay the prevalence of anti-HEV among Danish blood donors was 10.7% and with the Wantai assay the prevalence of anti-HEV was 19.8% (p < 0.001). In both cases the presence of anti-HEV was significantly correlated with increasing age. In addition, anti-HEV as measured by the Wantai test was significantly associated with contact with children (p = 0.01), but in multivariate analysis only age was associated with anti-HEV in both assays. By the NIH assay, the prevalence had declined from 20.6% in 2003 to 10.7% in 2013.
Anti-HEV prevalence had decreased by half among Danish blood donors over 10 years, but was still highly prevalent. The difference in reactivity of the two assays demonstrates the importance of using the same assay when comparing the anti-HEV prevalence in populations over time.
欧洲戊型肝炎（HEV）报告病例的发病率不断上升，这使人们将注意力集中在戊型肝炎病毒以及输血传播戊型肝炎的风险上。本研究的目的是调查2013年丹麦献血者中抗戊型肝炎病毒抗体（抗-HEV）的流行情况，并将其与丹麦以前的研究进行比较。此外，我们还想比较两种不同检测方法的相对反应性。
收集了504名献血者的样本，并用美国国立卫生研究院（NIH）开发的内部检测方法分析抗-HEV。此外，还用万泰抗-HEV检测方法对样本进行了分析。通过自行填写问卷收集人口统计学信息和可能的戊型肝炎病毒暴露情况。
使用NIH检测方法，丹麦献血者中抗-HEV的流行率为10.7%，而使用万泰检测方法，抗-HEV的流行率为19.8%（p<0.001）。在这两种情况下，抗-HEV的存在都与年龄增长显著相关。此外，万泰检测法测得的抗-HEV与接触儿童显著相关（p=0.01），但在多变量分析中，只有年龄在两种检测方法中都与抗-HEV相关。通过NIH检测法，流行率已从2003年的20.6%降至2013年的10.7%。
在10年时间里，丹麦献血者中抗-HEV的流行率下降了一半，但仍然很高。两种检测方法反应性的差异表明，在比较不同时期人群中抗-HEV流行率时，使用相同检测方法的重要性。
