Little Melissa H
The Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Victoria, Australia.
Curr Opin Genet Dev. 2015 Jun;32:135-43. doi: 10.1016/j.gde.2015.03.001. Epub 2015 Mar 26.
As with many mammalian organs, size and cellular complexity represent considerable challenges to the comprehensive analysis of kidney organogenesis. Traditional analyses in the mouse have revealed early patterning events and spatial cellular relationships. However, an understanding of later events is lacking. The generation of a comprehensive temporospatial atlas of gene expression during kidney development has facilitated advances in lineage definition, as well as selective compartment ablation. Advances in quantitative and dynamic imaging have allowed comprehensive analyses at the level of organ, component tissue and cell across kidney organogenesis. Such approaches will enhance our understanding of the links between kidney development and final postnatal organ function. The final frontier will be translating this understanding to outcomes for renal disease in humans.
与许多哺乳动物器官一样，大小和细胞复杂性对肾脏器官发生的全面分析构成了巨大挑战。对小鼠的传统分析揭示了早期的模式形成事件和空间细胞关系。然而，对后期事件的了解尚缺。肾脏发育过程中基因表达的全面时空图谱的生成促进了谱系定义以及选择性隔室消融方面的进展。定量和动态成像技术的进步使得在肾脏器官发生过程中对器官、组成组织和细胞水平进行全面分析成为可能。这些方法将增进我们对肾脏发育与出生后最终器官功能之间联系的理解。最终的挑战将是把这种理解转化为人类肾脏疾病的治疗成果。
