4-磷酸吡唑-2-基丙氨酸：磷酸组氨酸的一种不可水解类似物。
4-Phosphopyrazol-2-yl alanine: a non-hydrolysable analogue of phosphohistidine.
作者信息
Lilley Matthew, Mambwe Bezaleel, Thompson Mark J, Jackson Richard F W, Muimo Richmond
机构信息
Department of Chemistry, The University of Sheffield, Dainton Building, Sheffield, S3 7HF, UK.
出版信息
Chem Commun (Camb). 2015 Apr 30;51(34):7305-8. doi: 10.1039/c5cc01811k.
PMID:25820536
Abstract
We report the synthesis of a stable analogue of τ-phosphohistidine: 4-phosphopyrazol-2-yl alanine (pPza). Polyclonal antibodies generated against the mimic show high reactivity and selectivity for τ-phosphohistidine, with minor or no cross-reactivity towards non-phosphorylated histidine or O-phosphoamino acids, including phosphotyrosine.
摘要
我们报道了τ-磷酸组氨酸的一种稳定类似物：4-磷酸吡唑-2-基丙氨酸（pPza）的合成。针对该模拟物产生的多克隆抗体对τ-磷酸组氨酸表现出高反应性和选择性，对非磷酸化组氨酸或O-磷酸氨基酸（包括磷酸酪氨酸）的交叉反应较小或无交叉反应。
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