Ferretti V, Segal-Eiras Á, Barbeito C G, Croce M V
National University of La Plata.
Eur J Histochem. 2015 Feb 20;59(1):2462. doi: 10.4081/ejh.2015.2462.
Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during prenatal and postnatal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.
Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report which confirmed Muc5ac expression during skin development.
在人类胚胎和胎儿器官发育过程中已检测到一些黏蛋白基因；然而，关于黏蛋白在表皮发育中的表达，无论是在人类还是其他物种中，都知之甚少。本研究旨在探索产前和产后大鼠发育过程中Muc5ac在皮肤中的表达。采用了免疫组织化学（IHC）、蛋白质免疫印迹法（WB）和逆转录聚合酶链反应（RT-PCR）。通过免疫组织化学，在妊娠第13天至出生后7天的胚胎表皮中早期发现了Muc5ac蛋白，此时表面表皮呈阴性，反应局限于分泌皮脂的细胞。与免疫组织化学结果一致，蛋白质免疫印迹分析在相同发育时期显示出一条约200KDa的条带。逆转录聚合酶链反应也证实了结果。大鼠胚胎表皮中Muc5ac的表达表明，Muc5ac可能在出生前的胚胎皮肤中起保护作用，这种作用可能被绒毛覆盖所取代。据我们所知，这是第一份证实Muc5ac在皮肤发育过程中表达的报告。
大鼠胚胎表皮中Muc5ac的表达表明，Muc5ac可能在出生前的胚胎皮肤中起保护作用，这种作用可能被绒毛覆盖所取代。据我们所知，这是第一份证实Muc5ac在皮肤发育过程中表达的报告。
