Bălună R G, Barac D, Tarnavschi R, Belaşcu I
J Immunol Methods. 1985 May 10;79(1):65-70. doi: 10.1016/0022-1759(85)90392-8.
A hemagglutination assay for human serum fibronectin (Fn) is described. The assay is based on the capacity of Fn to bind to gelatin. Purified and human serum Fn agglutinate chromic chloride treated sheep red cells coated with gelatin in a quantitative and reproducible manner. The lowest Fn concentration giving positive hemagglutination was found to be 7 micrograms/ml, a sensitivity making it suitable for measurement of Fn in both normal and pathologic sera. The assay is simple and rapid and does not require specialized equipment.
本文描述了一种用于检测人血清纤连蛋白(Fn)的血凝试验。该试验基于Fn与明胶结合的能力。纯化的人血清Fn和人血清能够以定量且可重复的方式使包被有明胶的经氯化铬处理的绵羊红细胞发生凝集。产生阳性血凝反应的最低Fn浓度为7微克/毫升,这种灵敏度使其适用于检测正常和病理血清中的Fn。该试验简单快速,不需要专门的设备。