Roberts P L, Duncan B E, Raybould T J, Watson D H
J Gen Virol. 1985 May;66 ( Pt 5):1073-85. doi: 10.1099/0022-1317-66-5-1073.
Monoclonal antibodies which react with herpes simplex virus type 1 (HSV-1) glycoproteins, but do not neutralize infectivity, were prepared. The protein to which each monoclonal antibody was directed was determined by various techniques including their reaction with polypeptides from glycoprotein-deficient mutants after protein blotting, and also tests using passive haemagglutination. Monoclonal antibodies A7 and alpha C3 were directed against a type-specific determinant on gC and a type-common determinant on gB respectively. In addition, A7 reacted only with the HFEM strain of HSV-1 and did not react with any of the 20 low-passage human isolates also tested. The monoclonal antibodies were used in immunoadsorption chromatography to purify individual glycoproteins from detergent extracts of HSV-1-infected cells. The ability of the monoclonal antibodies or purified glycoproteins to protect mice against a lethal encephalitis induced by intracerebral inoculation of HSV-1 was investigated. Passive immunization was not very effective; however, purified gC or a mixture of gB and its precursor pgB induced good levels of neutralizing antibody which persisted for at least 9 weeks and mice survived virus challenge.
制备了与单纯疱疹病毒1型（HSV-1）糖蛋白发生反应但不中和感染性的单克隆抗体。通过多种技术确定了每种单克隆抗体所针对的蛋白质，包括蛋白质印迹后其与糖蛋白缺陷型突变体的多肽的反应，以及使用被动血凝试验进行的测试。单克隆抗体A7和αC3分别针对gC上的型特异性决定簇和gB上的型共同决定簇。此外，A7仅与HSV-1的HFEM株发生反应，而不与所测试的20株低代人分离株中的任何一株发生反应。这些单克隆抗体用于免疫吸附色谱法，从HSV-1感染细胞的去污剂提取物中纯化单个糖蛋白。研究了单克隆抗体或纯化的糖蛋白保护小鼠免受脑内接种HSV-1诱导的致死性脑炎的能力。被动免疫效果不太显著；然而，纯化的gC或gB及其前体pgB的混合物诱导产生了高水平的中和抗体，该抗体持续至少9周，并且小鼠在病毒攻击后存活下来。
