• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

染色质重塑因子RSC和ISW1表现出功能上以及基于染色质的启动子拮抗作用。

The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism.

作者信息

Parnell Timothy J, Schlichter Alisha, Wilson Boris G, Cairns Bradley R

机构信息

Department of Oncological Sciences, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, United States.

出版信息

Elife. 2015 Mar 30;4:e06073. doi: 10.7554/eLife.06073.

DOI:10.7554/eLife.06073
PMID:25821983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4423118/
Abstract

ISWI family chromatin remodelers typically organize nucleosome arrays, while SWI/SNF family remodelers (RSC) typically disorganize and eject nucleosomes, implying an antagonism that is largely unexplored in vivo. Here, we describe two independent genetic screens for rsc suppressors that yielded mutations in the promoter-focused ISW1a complex or mutations in the 'basic patch' of histone H4 (an epitope that regulates ISWI activity), strongly supporting RSC-ISW1a antagonism in vivo. RSC and ISW1a largely co-localize, and genomic nucleosome studies using rsc isw1 mutant combinations revealed opposing functions: promoters classified with a nucleosome-deficient region (NDR) gain nucleosome occupancy in rsc mutants, but this gain is attenuated in rsc isw1 double mutants. Furthermore, promoters lacking NDRs have the highest occupancy of both remodelers, consistent with regulation by nucleosome occupancy, and decreased transcription in rsc mutants. Taken together, we provide the first genetic and genomic evidence for RSC-ISW1a antagonism and reveal different mechanisms at two different promoter architectures.

摘要

ISWI家族染色质重塑因子通常会组织核小体阵列，而SWI/SNF家族重塑因子（RSC）通常会破坏并排出核小体，这意味着在体内存在一种很大程度上未被探索的拮抗作用。在这里，我们描述了针对rsc抑制因子的两项独立遗传筛选，结果在以启动子为重点的ISW1a复合物中产生了突变，或者在组蛋白H4的“碱性区域”（一个调节ISWI活性的表位）中产生了突变，这有力地支持了体内RSC-ISW1a的拮抗作用。RSC和ISW1a在很大程度上共定位，使用rsc isw1突变体组合进行的基因组核小体研究揭示了相反的功能：被归类为具有核小体缺陷区域（NDR）的启动子在rsc突变体中核小体占有率增加，但在rsc isw1双突变体中这种增加减弱。此外，缺乏NDR的启动子在两种重塑因子中占有率最高，这与核小体占有率的调节一致，并且在rsc突变体中转录减少。综上所述，我们提供了RSC-ISW1a拮抗作用的首个遗传和基因组证据，并揭示了两种不同启动子结构的不同机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/de5491780c98/elife06073f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/648c5facaa4f/elife06073f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/f98b3e0bcfd6/elife06073fs001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/10d932a1a308/elife06073f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/4836510b2781/elife06073fs002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/73f0b0d9ce6f/elife06073f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/9aa8b6b84da6/elife06073fs003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/0a58740b5e30/elife06073fs004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/45c785dec482/elife06073f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/62110927ff08/elife06073fs005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/6ac769e059a6/elife06073fs006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/b4f1da6c5c1d/elife06073f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/658f2ae0cc28/elife06073fs007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/d037ffaba41e/elife06073f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/d8665c5ef12e/elife06073fs008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/7806ecaffc63/elife06073fs009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/de5491780c98/elife06073f007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/648c5facaa4f/elife06073f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/f98b3e0bcfd6/elife06073fs001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/10d932a1a308/elife06073f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/4836510b2781/elife06073fs002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/73f0b0d9ce6f/elife06073f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/9aa8b6b84da6/elife06073fs003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/0a58740b5e30/elife06073fs004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/45c785dec482/elife06073f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/62110927ff08/elife06073fs005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/6ac769e059a6/elife06073fs006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/b4f1da6c5c1d/elife06073f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/658f2ae0cc28/elife06073fs007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/d037ffaba41e/elife06073f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/d8665c5ef12e/elife06073fs008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/7806ecaffc63/elife06073fs009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b05b/4423118/de5491780c98/elife06073f007.jpg

相似文献

1
The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism.染色质重塑因子RSC和ISW1表现出功能上以及基于染色质的启动子拮抗作用。
Elife. 2015 Mar 30;4:e06073. doi: 10.7554/eLife.06073.
2
Contrasting roles of the RSC and ISW1/CHD1 chromatin remodelers in RNA polymerase II elongation and termination.RSC 和 ISW1/CHD1 染色质重塑因子在 RNA 聚合酶 II 延伸和终止中的作用相反。
Genome Res. 2019 Mar;29(3):407-417. doi: 10.1101/gr.242032.118. Epub 2019 Jan 25.
3
ISWI and CHD chromatin remodelers bind promoters but act in gene bodies.ISWI 和 CHD 染色质重塑酶结合启动子，但在基因体内发挥作用。
PLoS Genet. 2013;9(2):e1003317. doi: 10.1371/journal.pgen.1003317. Epub 2013 Feb 28.
4
Chromatin remodeler Ino80C acts independently of H2A.Z to evict promoter nucleosomes and stimulate transcription of highly expressed genes in yeast.染色质重塑因子 Ino80C 独立于 H2A.Z 发挥作用，可驱逐启动子核小体并刺激酵母中高表达基因的转录。
Nucleic Acids Res. 2020 Sep 4;48(15):8408-8430. doi: 10.1093/nar/gkaa571.
5
The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening.RSC 染色质重塑复合物在酵母 PHO5 启动子打开的完整重塑复合物集中发挥着关键作用。
Nucleic Acids Res. 2014 Apr;42(7):4270-82. doi: 10.1093/nar/gkt1395. Epub 2014 Jan 24.
6
Chromatin remodelers clear nucleosomes from intrinsically unfavorable sites to establish nucleosome-depleted regions at promoters.染色质重塑因子从固有不利位置清除核小体，在启动子处建立核小体缺失区域。
Mol Biol Cell. 2011 Jun 15;22(12):2106-18. doi: 10.1091/mbc.E10-10-0826. Epub 2011 Apr 20.
7
SWI/SNF and RSC cooperate to reposition and evict promoter nucleosomes at highly expressed genes in yeast.SWI/SNF 和 RSC 合作将高度表达基因的启动子核小体重新定位并逐出。
Genes Dev. 2018 May 1;32(9-10):695-710. doi: 10.1101/gad.312850.118. Epub 2018 May 21.
8
Genome-wide nucleosome specificity and directionality of chromatin remodelers.染色质重塑因子的全基因组核小体特异性和方向性。
Cell. 2012 Jun 22;149(7):1461-73. doi: 10.1016/j.cell.2012.04.036.
9
Coordinated Action of Nap1 and RSC in Disassembly of Tandem Nucleosomes.Nap1和RSC在串联核小体解聚中的协同作用。
Mol Cell Biol. 2016 Aug 12;36(17):2262-71. doi: 10.1128/MCB.00195-16. Print 2016 Sep 1.
10
Specialization of the chromatin remodeler RSC to mobilize partially-unwrapped nucleosomes.染色质重塑因子 RSC 特异性地动员部分解旋核小体。
Elife. 2020 Jun 4;9:e58130. doi: 10.7554/eLife.58130.

引用本文的文献

1
Variations in flanking or less conserved positions of Reb1 and Abf1 consensus binding sites lead to major changes in their ability to modulate nucleosome sliding activity.Reb1和Abf1共有结合位点侧翼或保守性较低位置的变异，会导致它们调节核小体滑动活性能力的重大变化。
Biol Res. 2025 Jul 29;58(1):53. doi: 10.1186/s40659-025-00627-0.
2
General regulatory factors exert differential effects on nucleosome sliding activity of the ISW1a complex.一般调控因子对 ISW1a 复合物核小体滑动活性产生不同的影响。
Biol Res. 2024 May 4;57(1):22. doi: 10.1186/s40659-024-00500-6.
3
Enhancer switching in cell lineage priming is linked to eRNA, Brg1's AT-hook, and SWI/SNF recruitment.

本文引用的文献

1
RSC-dependent constructive and destructive interference between opposing arrays of phased nucleosomes in yeast.酵母中依赖RSC的相对相控核小体阵列之间的建设性和破坏性干扰。
Genome Res. 2014 Oct;24(10):1637-49. doi: 10.1101/gr.177014.114. Epub 2014 Jul 11.
2
The role of bromodomain proteins in regulating gene expression.溴结构域蛋白在调节基因表达中的作用。
Genes (Basel). 2012 May 29;3(2):320-43. doi: 10.3390/genes3020320.
3
Regulating the chromatin landscape: structural and mechanistic perspectives.调控染色质景观：结构与机制视角
细胞谱系启动中的增强子切换与 eRNA、Brg1 的 AT 钩和 SWI/SNF 募集有关。
Mol Cell. 2024 May 16;84(10):1855-1869.e5. doi: 10.1016/j.molcel.2024.03.013. Epub 2024 Apr 8.
4
Structure of the ISW1a complex bound to the dinucleosome.与双核小体结合的ISW1a复合物的结构。
Nat Struct Mol Biol. 2024 Feb;31(2):266-274. doi: 10.1038/s41594-023-01174-6. Epub 2024 Jan 4.
5
Energy-driven genome regulation by ATP-dependent chromatin remodellers.ATP 依赖的染色质重塑因子驱动的能量相关的基因组调控。
Nat Rev Mol Cell Biol. 2024 Apr;25(4):309-332. doi: 10.1038/s41580-023-00683-y. Epub 2023 Dec 11.
6
Poly(dA:dT) Tracts Differentially Modulate Nucleosome Remodeling Activity of RSC and ISW1a Complexes, Exerting Tract Orientation-Dependent and -Independent Effects.多聚(dA:dT)序列区差异调节 RSC 和 ISW1a 复合物的核小体重塑活性，发挥序列区取向依赖和非依赖效应。
Int J Mol Sci. 2023 Oct 17;24(20):15245. doi: 10.3390/ijms242015245.
7
ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.ISW1a 调节着着丝粒和着丝粒周围区域内黏连蛋白的分布。
Nucleic Acids Res. 2023 Sep 22;51(17):9101-9121. doi: 10.1093/nar/gkad612.
8
Differential requirements for Gcn5 and NuA4 HAT activities in the starvation-induced versus basal transcriptomes.饥饿诱导的转录组与基础转录组中 Gcn5 和 NuA4 HAT 活性的差异需求。
Nucleic Acids Res. 2023 May 8;51(8):3696-3721. doi: 10.1093/nar/gkad099.
9
The TRIPLE PHD FINGERS proteins are required for SWI/SNF complex-mediated +1 nucleosome positioning and transcription start site determination in Arabidopsis.TRIPLE PHD FINGERS 蛋白对于 SWI/SNF 复合物介导的 +1 核小体定位和转录起始位点在拟南芥中的确定是必需的。
Nucleic Acids Res. 2022 Oct 14;50(18):10399-10417. doi: 10.1093/nar/gkac826.
10
Histone protein surface accessibility dictates direction of RSC-dependent nucleosome mobilization.组蛋白表面可及性决定了依赖于 RSC 的核小体迁移的方向。
Nucleic Acids Res. 2022 Oct 14;50(18):10376-10384. doi: 10.1093/nar/gkac790.
Annu Rev Biochem. 2014;83:671-96. doi: 10.1146/annurev-biochem-051810-093157. Epub 2014 Mar 5.
4
The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening.RSC 染色质重塑复合物在酵母 PHO5 启动子打开的完整重塑复合物集中发挥着关键作用。
Nucleic Acids Res. 2014 Apr;42(7):4270-82. doi: 10.1093/nar/gkt1395. Epub 2014 Jan 24.
5
Mechanisms and functions of ATP-dependent chromatin-remodeling enzymes.ATP 依赖性染色质重塑酶的作用机制和功能。
Cell. 2013 Aug 1;154(3):490-503. doi: 10.1016/j.cell.2013.07.011.
6
A compendium of nucleosome and transcript profiles reveals determinants of chromatin architecture and transcription.核小体和转录谱摘要揭示了染色质结构和转录的决定因素。
PLoS Genet. 2013 May;9(5):e1003479. doi: 10.1371/journal.pgen.1003479. Epub 2013 May 2.
7
Heterochromatin protein Sir3 induces contacts between the amino terminus of histone H4 and nucleosomal DNA.异染色质蛋白 Sir3 诱导组蛋白 H4 的氨基末端与核小体 DNA 之间的接触。
Proc Natl Acad Sci U S A. 2013 May 21;110(21):8495-500. doi: 10.1073/pnas.1300126110. Epub 2013 May 6.
8
Regulation of nucleosome dynamics by histone modifications.组蛋白修饰调控核小体动力学。
Nat Struct Mol Biol. 2013 Mar;20(3):259-66. doi: 10.1038/nsmb.2470.
9
Functional antagonism between Sas3 and Gcn5 acetyltransferases and ISWI chromatin remodelers.Sas3 和 Gcn5 乙酰转移酶与 ISWI 染色质重塑酶之间的功能拮抗作用。
PLoS Genet. 2012;8(10):e1002994. doi: 10.1371/journal.pgen.1002994. Epub 2012 Oct 4.
10
Genome-wide nucleosome specificity and directionality of chromatin remodelers.染色质重塑因子的全基因组核小体特异性和方向性。
Cell. 2012 Jun 22;149(7):1461-73. doi: 10.1016/j.cell.2012.04.036.