Stanfield P R, Nakajima Y, Yamaguchi K
Nature. 1985;315(6019):498-501. doi: 10.1038/315498a0.
Much interest has recently centred on the properties of peptides that modulate the excitability of nerve cells. Such compounds include the undecapeptide substance P, which is particularly well established as an excitatory neurotransmitter, and we examine here its effects on magnocellular cholinergic neurones taken from the medial and ventral aspects of the globus pallidus of newborn rats and grown in dissociated culture. These neurones have previously been shown to respond to substance P3 and are analogous to the nucleus basalis of Meynert in man, which gives a diffuse projection to the cerebral cortex and whose degeneration is the likely cause of Alzheimer's disease. Substance P depolarizes these cultured neurones by reducing an inwardly rectifying potassium conductances; this conductance has been found in several neuronal types and has similar properties to those of certain other cells. As discussed below, modulation of inward (or anomalous) rectification by substance P implies a self-reinforcing element to the depolarization caused by the peptide.
最近，人们的许多兴趣都集中在调节神经细胞兴奋性的肽的特性上。这类化合物包括十一肽P物质，它作为一种兴奋性神经递质已得到充分证实。我们在此研究它对新生大鼠苍白球内侧和腹侧分离培养的大细胞胆碱能神经元的影响。这些神经元先前已被证明对P物质有反应，并且类似于人类的Meynert基底核，后者向大脑皮层发出弥散性投射，其退化可能是阿尔茨海默病的病因。P物质通过降低内向整流钾电导使这些培养的神经元去极化；这种电导已在几种神经元类型中发现，并且与某些其他细胞的电导具有相似的特性。如下所述，P物质对内向（或反常）整流的调节意味着该肽引起的去极化存在自我增强因素。
