Radbruch A, Zaiss S, Kappen C, Brüggemann M, Beyreuther K, Rajewsky K
Nature. 1985;315(6019):506-8. doi: 10.1038/315506a0.
In proliferating B lymphocytes, somatic mutation of rearranged antibody variable (V)-region genes occurs at high frequency and may have a key role in the selection of these cells. It is of interest in this context to learn in which way single mutations can affect antigen binding and/or idiotypic specificity of an antibody. Previous investigations have analysed spontaneous mutants of myeloma and hybridoma cells in which the mutation affected the antigen-binding specificity of the antibody. Here we describe an antibody mutant that has fully retained antigen-binding specificity but has lost or drastically changed all V-region antigenic determinants (idiotopes) of the wild type as defined by monoclonal anti-idiotope antibodies. The mutant phenotype is generated by a glycine to arginine exchange in the middle of the diversity (D) element, at position 103 of the heavy chain.
在增殖的B淋巴细胞中，重排的抗体可变（V）区基因的体细胞突变高频发生，并且可能在这些细胞的选择中起关键作用。在这种情况下，了解单个突变如何影响抗体的抗原结合和/或独特型特异性是很有意义的。先前的研究分析了骨髓瘤和杂交瘤细胞的自发突变体，其中突变影响了抗体的抗原结合特异性。在这里，我们描述了一种抗体突变体，它完全保留了抗原结合特异性，但失去或显著改变了野生型的所有V区抗原决定簇（独特型），这是由单克隆抗独特型抗体定义的。突变表型是由重链第103位多样性（D）元件中间的甘氨酸到精氨酸交换产生的。
