独特型表位的化学合成。针对同一JH1肽的抗血清可检测多个与结合位点相关的独特型表位的证据。
Chemical synthesis of idiotopes. Evidence that antisera to the same JH1 peptide detect multiple binding site-associated idiotopes.
作者信息
Seiden M V, Clevinger B, McMillan S, Srouji A, Lerner R, Davie J M
出版信息
J Exp Med. 1984 May 1;159(5):1338-50. doi: 10.1084/jem.159.5.1338.
Abstract
In an attempt to better understand the molecular basis of idiotypy, we have generated several site-specific antisera through immunization of animals with synthetic peptides corresponding to the (JH1) heavy chain joining segment 1 of the mouse heavy chain variable (VH) region. These anti-peptide sera identify several idiotypic determinants present on intact hybridoma and myeloma immunoglobulins. Expression of at least three of these idiotopes is correlated with the antigen specificity of the family of immunoglobulins bearing the determinant. Use of synthetic peptides may prove a powerful technique in the generation of molecularly defined antiidiotypic reagents.
摘要
为了更好地理解个体基因型的分子基础，我们通过用与小鼠重链可变区（VH）的（JH1）重链连接段1相对应的合成肽免疫动物，产生了几种位点特异性抗血清。这些抗肽血清识别完整杂交瘤和骨髓瘤免疫球蛋白上存在的几种个体基因型决定簇。这些个体基因型表位中至少有三种的表达与带有该决定簇的免疫球蛋白家族的抗原特异性相关。合成肽的使用可能被证明是一种在产生分子定义的抗个体基因型试剂方面的强大技术。
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Chemical synthesis of idiotopes. Evidence that antisera to the same JH1 peptide detect multiple binding site-associated idiotopes.独特型表位的化学合成。针对同一JH1肽的抗血清可检测多个与结合位点相关的独特型表位的证据。
J Exp Med. 1984 May 1;159(5):1338-50. doi: 10.1084/jem.159.5.1338.
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