Gol S, Ros-Freixedes R, Zambonelli P, Tor M, Pena R N, Braglia S, Zappaterra M, Estany J, Davoli R
Departament de Producció Animal-Agrotecnio Center, Universitat de Lleida, Lleida, Catalonia, Spain.
Department of Agricultural and Food Science (DISTAL), University of Bologna, Reggio Emilia, Italy.
J Anim Breed Genet. 2016 Feb;133(1):24-30. doi: 10.1111/jbg.12159. Epub 2015 Mar 30.
The perilipins (PLIN) belong to a family of structural proteins that play a role regulating intracellular lipid storage and mobilization. Here, PLIN1 and PLIN2 have been evaluated as candidate genes for growth, carcass and meat quality traits in pigs. A sample of 607 Duroc pigs were genotyped for two single-nucleotide polymorphisms, one in intron 2 of the PLIN1 gene (JN860199:g.173G>A) and the other at the 3' untranslated region of the PLIN2 gene (GU461317:g.98G>A). Using a Bayesian approach, we have been able to find evidence of additive, dominant and epistatic associations of the PLIN1 and PLIN2 polymorphisms with early growth rate and carcass length. However, the major effects were produced by the dominant A allele at the PLIN2 polymorphism, which also affected the carcass lean weight. Thus, pigs carrying an additional copy of the A allele at the g.98G>A PLIN2 polymorphism had a probability of at least 98% of producing carcasses with heavier lean weight (+0.41 kg) and ham weight (+0.10 kg). The results obtained indicate that the PLIN2 polymorphism could be a useful marker for lean growth. In particular, it may help to reduce the undesired negative correlated response in lean weight to selection for increased intramuscular fat content, a common scenario in some Duroc lines involved in the production of high quality pork products.
perilipins（PLIN）属于一类结构蛋白家族，在调节细胞内脂质储存和动员方面发挥作用。在此，PLIN1和PLIN2已被评估为猪生长、胴体和肉质性状的候选基因。对607头杜洛克猪的样本进行了两个单核苷酸多态性的基因分型，一个在PLIN1基因的内含子2中（JN860199:g.173G>A），另一个在PLIN2基因的3'非翻译区（GU461317:g.98G>A）。使用贝叶斯方法，我们能够找到PLIN1和PLIN2多态性与早期生长速度和胴体长度的加性、显性和上位性关联的证据。然而，主要效应是由PLIN2多态性的显性A等位基因产生的，它也影响胴体瘦肉重。因此，在g.98G>A PLIN2多态性处携带额外一个A等位基因拷贝的猪，生产出瘦肉重更重（+0.41千克）和火腿重更重（+0.10千克）胴体的概率至少为98%。获得的结果表明，PLIN2多态性可能是瘦肉生长的一个有用标记。特别是，它可能有助于减少在一些参与生产高品质猪肉产品的杜洛克品系中，对肌肉内脂肪含量增加进行选择时，瘦肉重中不期望的负相关反应。
