日本腹泻犬中犬星状病毒的检测

Takano Tomomi, Takashina Midori, Doki Tomoyoshi, Hohdatsu Tsutomu

Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori, 034-8628, Japan.

Arch Virol. 2015 Jun;160(6):1549-53. doi: 10.1007/s00705-015-2405-3. Epub 2015 Apr 1.

Canine astrovirus (CAstV) is the causative agent of gastroenteritis in dogs. We collected rectal swabs from dogs with or without diarrhea symptoms in Japan and examined the feces for the presence of CAstV by RT-PCR with primers based on a conserved region of the ORF1b gene. The ORF1b gene of CAstV was not detected in the 42 dogs without clinical illness but was present in three pups out of the 31 dogs with diarrhea symptoms. Based on the full-length capsid protein, the CAstV KU-D4-12 strain that we detected in this study shared high homology with the novel virulent CAstV VM-2011 strain.

犬星状病毒（CAstV）是犬类肠胃炎的病原体。我们在日本采集了有或无腹泻症状犬的直肠拭子，并使用基于ORF1b基因保守区域的引物通过逆转录聚合酶链反应（RT-PCR）检测粪便中是否存在CAstV。在42只无临床疾病的犬中未检测到CAstV的ORF1b基因，但在31只有腹泻症状的幼犬中有3只检测到该基因。基于全长衣壳蛋白，我们在本研究中检测到的CAstV KU-D4-12毒株与新型强毒株CAstV VM-2011毒株具有高度同源性。