Jafari Rasool, Maghsood Amir Hossein, Safari Marzieh, Latifi Milad, Fallah Mohammad
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran.
Jundishapur J Microbiol. 2015 Feb 2;8(2):e16470. doi: 10.5812/jjm.16470. eCollection 2015 Feb.
Fecal antigen detection using the enzyme linked immunosorbent assay (ELISA) and oocyst detection using auramine phenol (AP) staining methods, are told to be more sensitive compared to other conventional methods, for diagnosis of cryptosporidiosis.
The aim of the present study was to evaluate the antigen-detection capacity in the stool specimens using ELISA and oocyst detection by AP staining methods, for the diagnosis of human cryptosporidiosis.
A total of 228 fecal samples were collected from residents of rural areas of Hamadan, West of Iran. Each fecal sample was divided into two parts, one kept frozen at -20˚C for Ag-capture ELISA and the other in 10% formalin for the AP staining method. Cryptosporidium Ag-detection ELISA procedure was performed according to the manual of the manufacturer. The preserved samples concentrated using the formalin-ether concentration technique were stained with AP and then investigated under florescent microscopy.
Eight (3.5%) and three (1.3%) out of 228 fecal samples were positive for Cryptosporidium infection by ELISA and AP staining methods, respectively. Cryptosporidium Ag-detection using ELISA showed an increased frequency of the infection, compared to the AP staining method (P = 0.062).
For epidemiological studies and diagnostic purposes of the Cryptosporidium infection, especially in asymptomatic individuals, Ag-detection ELISA is an easy to perform and accurate method, compared to other conventional microscopic methods.
酶联免疫吸附测定（ELISA）法检测粪便抗原以及金胺酚（AP）染色法检测卵囊，在隐孢子虫病诊断中据说是比其他传统方法更敏感。
本研究旨在评估使用ELISA法检测粪便标本中的抗原以及用AP染色法检测卵囊在诊断人体隐孢子虫病方面的能力。
从伊朗西部哈马丹农村地区居民中总共收集了228份粪便样本。每份粪便样本分为两部分，一部分保存在-20˚C用于抗原捕获ELISA检测，另一部分保存在10%福尔马林中用于AP染色法检测。隐孢子虫抗原检测ELISA程序按照制造商手册进行。用福尔马林-乙醚浓缩技术浓缩保存的样本，用AP染色，然后在荧光显微镜下检查。
228份粪便样本中，分别有8份（3.5%）和3份（1.3%）通过ELISA法和AP染色法检测出隐孢子虫感染呈阳性。与AP染色法相比，使用ELISA法检测隐孢子虫抗原显示感染频率增加（P = 0.062）。
对于隐孢子虫感染的流行病学研究和诊断目的，特别是在无症状个体中，与其他传统显微镜检查方法相比，抗原检测ELISA是一种易于操作且准确的方法。
