Razakandrainibe Romy, Mérat Célia, Kapel Nathalie, Sautour Marc, Guyot Karine, Gargala Gilles, Ballet Jean-Jacques, Le Pape Patrice, Dalle Frédéric, Favennec Loïc
EA ESCAPE 7510, University of Medicine Pharmacy Rouen, 76000 Rouen, France.
CNR LE Cryptosporidiosis, Santé Publique France, 76000 Rouen, France.
Microorganisms. 2021 Jan 20;9(2):209. doi: 10.3390/microorganisms9020209.
Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA kit, Savyon Diagnostics, Israel) for the detection of spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.
人类隐孢子虫病的诊断仍不足，快速/准确的诊断具有临床重要性。通过传统显微镜检查粪便样本中的卵囊进行诊断，劳动强度大、耗时且需要熟练的经验。因此，我们旨在评估粪便抗原酶联免疫吸附测定（ELISA）试验在检测粪便标本中隐孢子虫属的实用性。为此，我们通过一项多中心研究评估了一种用于检测随机临床粪便样本中隐孢子虫属的商业ELISA（CoproELISA试剂盒，以色列Savyon诊断公司）的性能。粪便抗原ELISA的敏感性和特异性分别为98.86%和94.32%。粪便抗原ELISA结果表明，这种简单、快速、可靠且标准化的免疫测定试验对常规诊断敏感且特异，可能有助于隐孢子虫病的大规模流行病学研究。
