Abuelba Hussam, Cotrutz Carmen Elena, Stoica Bogdan Alexandru, Stoica Laura, Olinici DoiniŢa, Petreuş Tudor
Department of Cell and Molecular Biology, "Grigore T. Popa" University of Medicine and Pharmacy, Iassy, Romania;
Rom J Morphol Embryol. 2015;56(1):71-6.
Breast adenocarcinoma cell line MDA-MB-231, even if it expresses low levels of E-cadherin, still readily form multicellular aggregates of cells, namely spheroids. Curcumin is a diarylheptanoid antitumoral drug while it significantly inhibits cell migration, invasion, and colony formation in vitro and reduces tumor growth and liver metastasis in vivo. Curcumin photoactivation may enhance antiapoptotic role against cancer cells.
To evaluate the effect of low curcumin concentrations, ranged from 1.9 to 15 μM, with and without photoactivation, using a manufactured 670 nm LED-matrix. A secondary aim was to evaluate the ideal method to produce easy-to-use tumor cell spheroids, comparing two low adherence plate supports.
Breast adenocarcinoma cell line MDA-MB-231 were cultured according to 2D monolayer and 3D spheroid models then submitted to normal and photoactivated curcumin in micromolar concentrations. MTT assay was used to evaluate cell viability following curcumin application on cells. On 2D cell cultures, curcumin inhibits cell tumor development and proliferation at concentrations of 15 μM, with a viability of 65.7% at 48 hours incubation time. A decreased viability up to 25% for a concentration of 15 μM was recorded following photoactivation and cytotoxic action on breast cancer tumor cell line continued at concentrations of 7.5 and 3.75 μM. Curcumin photoactivation increases pro-apoptotic effects in both 2D and 3D tumor cell culture models and also responsiveness to curcumin is slightly reduced in spheroid-like structures. Thus, 3D tumor cell culture systems appear to be the ideal environment for in vitro assays regarding anticancer drug effects on cell viability.
乳腺癌细胞系MDA-MB-231即使表达低水平的E-钙黏蛋白，仍易于形成多细胞聚集体，即球体。姜黄素是一种二芳基庚烷类抗肿瘤药物，它在体外能显著抑制细胞迁移、侵袭和集落形成，并在体内减少肿瘤生长和肝转移。姜黄素光活化可能增强对癌细胞的抗凋亡作用。
使用制造的670 nm LED矩阵，评估1.9至15 μM低浓度姜黄素在有无光活化情况下的效果。第二个目的是比较两种低粘附平板支架，评估生产易于使用的肿瘤细胞球体的理想方法。
乳腺癌细胞系MDA-MB-231按照二维单层和三维球体模型培养，然后用微摩尔浓度的正常姜黄素和光活化姜黄素处理。MTT法用于评估姜黄素作用于细胞后的细胞活力。在二维细胞培养中，姜黄素在15 μM浓度下抑制细胞肿瘤发展和增殖，在孵育48小时时活力为65.7%。光活化后，15 μM浓度下的活力降低至25%，对乳腺癌肿瘤细胞系的细胞毒性作用在7.5和3.75 μM浓度下持续存在。姜黄素光活化在二维和三维肿瘤细胞培养模型中均增加促凋亡作用，并且在类球体结构中对姜黄素的反应性略有降低。因此，三维肿瘤细胞培养系统似乎是用于体外检测抗癌药物对细胞活力影响的理想环境。
