松蕈酸对棕榈酸诱导的C2C12肌管脂毒性的细胞保护作用。

Cytoprotective effect of hispidin against palmitate-induced lipotoxicity in C2C12 myotubes.

作者信息

Park Jun Myoung, Lee Jong Seok, Song Jeong Eun, Sim Ye Chan, Ha Suk-Jin, Hong Eock Kee

机构信息

Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon-do 200-701, Korea.

National Institute of Biological Resources, Incheon 404-708, Korea.

出版信息

Molecules. 2015 Mar 27;20(4):5456-67. doi: 10.3390/molecules20045456.

It is well known that Phellinus linteus, which produces hispidin and its derivatives, possesses antioxidant activities. In this study, we investigated whether hispidin has protective effects on palmitate-induced oxidative stress in C2C12 skeletal muscle cells. Our results showed that palmitate treatment in C2C12 myotubes increased ROS generation and cell death as compared with the control. However, pretreatment of hispidin for 8 h improved the survival of C2C12 myotubes against palmitate-induced oxidative stress via inhibition of intracellular ROS production. Hispidin also inhibited palmitate-induced apoptotic nuclear condensation in C2C12 myotubes. In addition, we found that hispidin can suppress cleavage of caspase-3, expression of Bax, and NF-κB translocation. Therefore, these results suggest that hispidin is capable of protecting C2C12 myotubes against palmitate-induced oxidative stress.

众所周知，产生漆斑菌素及其衍生物的桑黄具有抗氧化活性。在本研究中，我们调查了漆斑菌素对棕榈酸酯诱导的C2C12骨骼肌细胞氧化应激是否具有保护作用。我们的结果表明，与对照组相比，C2C12肌管中的棕榈酸酯处理增加了活性氧的产生和细胞死亡。然而，漆斑菌素预处理8小时通过抑制细胞内活性氧的产生，提高了C2C12肌管对棕榈酸酯诱导的氧化应激的存活率。漆斑菌素还抑制了C2C12肌管中棕榈酸酯诱导的凋亡核浓缩。此外，我们发现漆斑菌素可以抑制caspase-3的裂解、Bax的表达和NF-κB的易位。因此，这些结果表明漆斑菌素能够保护C2C12肌管免受棕榈酸酯诱导的氧化应激。