Department of Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Islamic Republic of Iran.
Department of Clinical Biochemistry, Faculty of Medicine Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
Inflammopharmacology. 2018 Oct;26(5):1265-1272. doi: 10.1007/s10787-018-0466-0. Epub 2018 Apr 11.
Curcumin, a natural polyphenol compound, has the beneficial effects on several diseases such as metabolic syndrome, cancer, and diabetes. The anti-inflammatory property of curcumin has been demonstrated in different cells; however, its role in prevention of palmitate-induced inflammation in skeletal muscle C2C12 cells is not known. In this study, we examined the effect of curcumin on the inflammatory responses stimulated by palmitate in C2C2 cells. The results showed that palmitate upregulated the mRNA expression and protein release of IL-6 and TNF-α cytokines in C2C12 cells, while pretreatment with curcumin was able to attenuate the effect of palmitate on inflammatory cytokines. The anti-inflammatory effect of curcumin was associated with the repression of phosphorylation of IKKα-IKKβ, and JNK. Palmitate also caused an increase in reactive oxygen species (ROS) level that curcumin abrogated it. Collectively, these findings suggest that curcumin may represent a promising therapy for prevention of inflammation in skeletal muscle cells.
姜黄素是一种天然多酚化合物，对代谢综合征、癌症和糖尿病等多种疾病有有益作用。姜黄素的抗炎特性已在不同细胞中得到证实；然而，其在预防棕榈酸诱导的骨骼肌 C2C12 细胞炎症中的作用尚不清楚。在这项研究中，我们研究了姜黄素对 C2C2 细胞中棕榈酸盐刺激的炎症反应的影响。结果表明，棕榈酸上调了 C2C12 细胞中白细胞介素-6 和肿瘤坏死因子-α 细胞因子的 mRNA 表达和蛋白释放，而姜黄素预处理能够减弱棕榈酸对炎症细胞因子的作用。姜黄素的抗炎作用与 IKKα-IKKβ 和 JNK 的磷酸化抑制有关。棕榈酸还导致活性氧 (ROS) 水平增加，而姜黄素则消除了这一作用。总之，这些发现表明姜黄素可能是预防骨骼肌细胞炎症的一种有前途的治疗方法。
