定位单核小体的制备与分析
Preparation and analysis of positioned mononucleosomes.
作者信息
Kulaeva Olga I, Studitsky Vasily M
机构信息
Cancer Epigenetics Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA.
出版信息
Methods Mol Biol. 2015;1288:15-26. doi: 10.1007/978-1-4939-2474-5_2.
Abstract
Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200-350-bp DNA fragments.
摘要
含有单核小体的短DNA片段已被广泛用作简单的模型实验系统，用于分析许多核内过程，包括蛋白质与核小体的结合、组蛋白共价修饰、转录、DNA修复以及ATP依赖的染色质重塑。在此，我们描述了几种最近开发的用于获取和分析组装在200 - 350碱基对DNA片段上的单核小体的方法。
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