用于体外功能测定的修饰染色质模板的重构。
Reconstitution of modified chromatin templates for in vitro functional assays.
作者信息
Yun Miyong, Ruan Chun, Huh Jae-Wan, Li Bing
机构信息
Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX, USA.
出版信息
Methods Mol Biol. 2012;833:237-53. doi: 10.1007/978-1-61779-477-3_15.
Abstract
To study the functions of histone modifications in the context of chromatin, it is necessary to be able to prepare nucleosomal templates that carry specific posttranslational modifications in a defined biochemical system. Here, we describe two sets of protocols for reconstituting designer nucleosomes that contain specifically modified histones. The resulting nucleosomes are suitable for electromobility shift assays, chromatin remodeling assays, and other functional and structural studies.
摘要
为了在染色质环境中研究组蛋白修饰的功能，必须能够在特定的生化系统中制备携带特定翻译后修饰的核小体模板。在此，我们描述了两组用于重构含有特定修饰组蛋白的定制核小体的方案。所得核小体适用于电泳迁移率变动分析、染色质重塑分析以及其他功能和结构研究。
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