Wang Yun-Xiang, Huang Fa-Qi, Nogi Yuichi, Pang Shou-Ji, Wang Ping-Kang, Lv Jie
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2048-2055. doi: 10.1099/ijs.0.000219. Epub 2015 Apr 1.
A Gram-reaction-negative bacterial strain, designated fig4(T), was isolated from a subsurface sediment core of Qiangtang Basin permafrost in China. Cells were catalase- and oxidase-positive and rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain fig4(T )was a member of the family Hyphomicrobiaceae and was most closely related to members of the genera Pelagibacterium, Vasilyevaea and Devosia with 93.8-96.2% sequence similarities. The major cellular fatty acids were C16 : 0, C18 : 0, 11-methyl C18 : 1 ω7c, C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The major respiratory quinone was Q-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The DNA G+C content was 60.7 mol%. Based on the phenotypic, phylogenetic and genotypic data, strain fig4(T) is considered to represent a novel species of a new genus in the family Hyphomicrobiaceae, for which the name Youhaiella tibetensis gen. nov., sp. nov. is proposed. The type strain is fig4(T) ( = CGMCC 1.12719(T) = JCM 19854(T)).
从中国羌塘盆地多年冻土的地下沉积物岩芯中分离出一株革兰氏反应阴性细菌，命名为fig4(T)。细胞过氧化氢酶和氧化酶呈阳性，为杆状。基于16S rRNA基因序列的系统发育分析表明，菌株fig4(T)是生丝微菌科的成员，与Pelagibacterium属、Vasilyevaea属和Devosia属的成员关系最为密切，序列相似性为93.8 - 96.2%。主要细胞脂肪酸为C16 : 0、C18 : 0、11 - 甲基C18 : 1 ω7c、C19 : 0环ω8c和总和特征8（C18 : 1ω7c和/或C18 : 1ω6c）。主要呼吸醌为Q - 10，主要极性脂为双磷脂酰甘油、磷脂酰甘油和两种未知糖脂。DNA G + C含量为60.7 mol%。基于表型、系统发育和基因型数据，菌株fig4(T)被认为代表生丝微菌科一个新属的一个新物种，为此提出新属名Youhaiella tibetensis gen. nov., sp. nov.。模式菌株为fig4(T)（= CGMCC 1.12719(T) = JCM 19854(T)）。
