Kim Hyun-Sook, Srinivasan Sathiyaraj, Lee Sang-Seob
Department of Bioengineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
Department of Life Science, College of Natural Science, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Jan;66(1):101-106. doi: 10.1099/ijsem.0.000680. Epub 2015 Oct 14.
A Gram-stain-negative, aerobic, short rod-shaped and motile bacterial strain, designated M48T, was isolated from a soil sample contaminated with chloroethylene compounds. Strain M48T was able to biodegrade methanol as a sole carbon source. Based on 16S rRNA gene sequences, strain M48T was closely related to Devosia chinhatensis IPL18T, Devosia insulae DS-56T, Vasilyevaea enhydra 9bT and Devosia psychrophila Cr7-05T, showing sequence similarities of 96.0, 95.8, 95.6 and 95.5 %, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M48T formed a distinct lineage within the family Hyphomicrobiaceae of the class Alphaproteobacteria. Strain M48T contains Q-10 as the predominant ubiquinone, and C18 : 1ω7c 11-metyl, C16 : 0, C19 : 0 cyclo ω8c and C18 : 1ω7c as the major fatty acids. The polar lipid profile includes diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and dimannosyldiacylglycerol. The DNA G+C content of strain M48T was 60.3 mol%. Based on phenotypic, phylogenetic and chemotaxonomic data, strain M48T represents a novel species of a new genus within the family Hyphomicrobiaceae, for which the name Methyloterrigena soli gen. nov., sp. nov. is proposed. The type strain of the type species is M48T ( = KEMB 224-262T = JCM 30821T).
从受氯乙烯化合物污染的土壤样本中分离出一株革兰氏染色阴性、需氧、短杆状且具运动性的细菌菌株，命名为M48T。菌株M48T能够以甲醇作为唯一碳源进行生物降解。基于16S rRNA基因序列，菌株M48T与中华德沃斯氏菌IPL18T、岛屿德沃斯氏菌DS-56T、水栖瓦西里耶夫氏菌9bT和嗜冷德沃斯氏菌Cr7-05T密切相关，序列相似性分别为96.0%、95.8%、95.6%和95.5%。基于16S rRNA基因序列的系统发育分析表明，菌株M48T在α-变形菌纲生丝微菌科内形成一个独特的分支。菌株M48T以Q-10作为主要泛醌，以C18 : 1ω7c 11-甲基、C16 : 0、C19 : 0环ω8c和C18 : 1ω7c作为主要脂肪酸。极性脂质谱包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和二甘露糖二酰基甘油。菌株M48T的DNA G+C含量为60.3 mol%。基于表型、系统发育和化学分类学数据，菌株M48T代表生丝微菌科内一个新属的新物种，为此提出新属名为甲基土生菌属（Methyloterrigena），新种名为土壤甲基土生菌（Methyloterrigena soli）。模式种的模式菌株为M48T（=KEMB 224-262T =JCM 30821T）。
