Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, People's Republic of China.
Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, People's Republic of China.
Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1817-1822. doi: 10.1099/ijs.0.023325-0. Epub 2010 Sep 3.
Two Gram-negative, motile, aerobic bacterial strains, designated B2(T) and 1_C16_27(T), were respectively isolated from a seawater sample collected from the East China Sea and a semi-coke sample from north-eastern Estonia. Their genetic, phenotypic and chemotaxonomic properties were studied. The isolates were short rods with polar flagella and were positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The major fatty acids were nonadecanoic (C(19 : 0) cyclo), octadecanoic (C(18 : 0) and C(18 : 0) 3-OH), octadecenoic (C(18 : 1)) and hexadecanoic (C(16 : 0)) acids. The G+C content of the genomic DNA was 58.1-59.3 mol%. 16S rRNA gene sequence analysis revealed that the two isolates represent a distinct lineage within the family Hyphomicrobiaceae. The phylogenetically closest relatives were Cucumibacter (92.7-93.7 % 16S rRNA gene sequence similarity), Devosia (92.9-94.4 %) and Zhangella (91.7-92.1 %). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strains B2(T) and 1_C16_27(T) could be differentiated from each other and from members of the genera Cucumibacter, Devosia and Zhangella. Therefore, it is proposed that strains B2(T) and 1_C16_27(T) represent two novel species in a new genus, for which the names Pelagibacterium halotolerans gen. nov., sp. nov. (the type species; type strain B2(T) = CGMCC 1.7692(T) = JCM 15775(T)) and Pelagibacterium luteolum sp. nov. (type strain 1_C16_27(T) = CGMCC 1.10267(T) = JCM 16552(T) = CELMS EEUT 1C1627(T)) are proposed.
两株革兰氏阴性、运动、需氧细菌菌株，分别命名为 B2(T) 和 1_C16_27(T)，分别从东海海水样本和来自东北爱沙尼亚的半焦样本中分离得到。研究了它们的遗传、表型和化学分类学特性。这些分离物是具有极性鞭毛的短杆菌，过氧化氢酶和氧化酶活性均为阳性。Q-10 是主要的呼吸泛醌。主要的极性脂是磷脂酰甘油、双磷脂酰甘油和两种未鉴定的糖脂。主要脂肪酸是非十九烷酸（C(19 : 0) 环）、十八烷酸（C(18 : 0) 和 C(18 : 0) 3-OH）、十八烯酸（C(18 : 1)）和十六烷酸（C(16 : 0)）。基因组 DNA 的 G+C 含量为 58.1-59.3 mol%。16S rRNA 基因序列分析表明，这两个分离物代表了噬氢菌科中的一个独特谱系。与亲缘关系最近的是 Cucumibacter（92.7-93.7% 16S rRNA 基因序列相似性）、Devosia（92.9-94.4%）和 Zhangella（91.7-92.1%）。表型差异特征以及系统发育和遗传独特性表明，菌株 B2(T) 和 1_C16_27(T) 彼此之间以及与 Cucumibacter、Devosia 和 Zhangella 属的成员都可以区分开来。因此，建议菌株 B2(T) 和 1_C16_27(T) 代表一个新属中的两个新种，该属命名为 Pelagibacterium halotolerans gen. nov.，sp. nov.（模式种；模式菌株 B2(T) = CGMCC 1.7692(T) = JCM 15775(T)) 和 Pelagibacterium luteolum sp. nov.（模式菌株 1_C16_27(T) = CGMCC 1.10267(T) = JCM 16552(T) = CELMS EEUT 1C1627(T)）。
