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使用基于DNA的检测方法检测肉类产品中的标签错误情况。

Occurrence of mislabeling in meat products using DNA-based assay.

作者信息

Di Pinto Angela, Bottaro Marilisa, Bonerba Elisabetta, Bozzo Giancarlo, Ceci Edmondo, Marchetti Patrizia, Mottola Anna, Tantillo Giuseppina

机构信息

Department of Veterinary Medicine, University of Bari Aldo Moro, Prov. le Casamassima, km 3, 70010 Valenzano (Bari), Italy.

出版信息

J Food Sci Technol. 2015 Apr;52(4):2479-84. doi: 10.1007/s13197-014-1552-y. Epub 2014 Sep 17.

DOI:10.1007/s13197-014-1552-y
PMID:25829637
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4375227/
Abstract

Considering that the authentication of food contents is one of the most important issues for the food quality sector, and given the increasing demand for transparency in the meat industry followed the horsemeat scandal in Europe, this study investigates processed-meat products from Italian markets and supermarkets using the mitochondrial cytochrome b gene qualitative PCR identification system in order to verify any species substitution or mislabeling. The results revealed a high substitution rate among the meat products, highlighting a mislabeling rate of 57 %, and consequently, considerable discordance with the indications on the labels, which raises significant food-safety and consumer-protection concerns.

摘要

鉴于食品成分的鉴定是食品质量领域最重要的问题之一,并且鉴于欧洲马肉丑闻后肉类行业对透明度的需求不断增加,本研究使用线粒体细胞色素b基因定性PCR鉴定系统对意大利市场和超市的加工肉类产品进行调查,以核实是否存在任何物种替代或标签错误的情况。结果显示,肉类产品中的替代率很高,突出显示标签错误率为57%,因此,与标签上的说明存在相当大的不一致,这引发了重大的食品安全和消费者保护问题。

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本文引用的文献

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Molecular assay to fraud identification of meat products.分子检测技术在肉类产品掺假鉴别中的应用。
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DNA barcoding for detecting market substitution in salted cod fillets and battered cod chunks.利用 DNA 条码技术检测腌制鳕鱼鱼片和面糊鳕鱼块中的市场替代物。
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A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products.一种用于检测和定量加工禽肉产品中猪肉含量的 SYBR Green 实时 PCR 检测方法。
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Food authentication of commercially-relevant shrimp and prawn species: from classical methods to Foodomics.商业相关虾类和对虾的食品鉴定:从经典方法到食品组学。
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Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction.TaqMan 探针实时聚合酶链反应检测商业肉丸中的猪肉掺假,针对猪特异性线粒体细胞色素 b 基因。
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