Klein Nathan D, Hurley Katie R, Feng Z Vivian, Haynes Christy L
†University of Minnesota, Department of Chemistry, 207 Pleasant St. SE, Minneapolis, Minnesota 55455, United States.
‡Augsburg College, Department of Chemistry, 2211 Riverside Ave., Minneapolis, Minnesota 55454, United States.
Anal Chem. 2015 Apr 21;87(8):4356-62. doi: 10.1021/acs.analchem.5b00124. Epub 2015 Apr 9.
Dark field transmission electron microscopy has been applied herein to visualize the interactions of inorganic nanomaterials with biological systems. This new application of a known technique addresses a deficiency in status quo visualization techniques. High resolution and low noise images can be acquired to locate and identify crystalline nanoparticles in complex biological matrices. Moreover, through the composition of multiple images taken at different angular beam tilts, it is possible to image a majority of nanoparticles present at a site in dark field mode. This facilitates clarity regarding the internalization of nanomaterials in cellular systems. In addition, comparing dark field images recorded at different angular tilts yields insight into the character of nanoparticle faceting.
本文应用暗场透射电子显微镜来观察无机纳米材料与生物系统的相互作用。这项已知技术的新应用弥补了现有可视化技术的不足。可以获取高分辨率、低噪声的图像，以在复杂的生物基质中定位和识别结晶纳米颗粒。此外，通过组合在不同角度束倾斜下拍摄的多张图像，能够以暗场模式对存在于某一位置的大多数纳米颗粒进行成像。这有助于清晰了解纳米材料在细胞系统中的内化情况。此外，比较在不同角度倾斜下记录的暗场图像，有助于深入了解纳米颗粒刻面的特征。
