Xie Zongtao, Cai Liming, Li Runsheng, Zheng Jinyu, Wu Hongyan, Yang Xiaoqi, Li Hu, Wang Zhiqiang
Department of Thoracic and Cardiovascular Surgery, Affiliated Hospital of Jiangnan University, The Forth People's Hospital of Wuxi City, No. 200, Huihe Road, Wuxi, 214062, China.
Tumour Biol. 2015 Aug;36(8):6497-505. doi: 10.1007/s13277-015-3340-3. Epub 2015 Apr 2.
microRNAs are small, non-coding RNAs that contribute into various biological processes during cancer progression. However, the potential role of miR-489 in the development of Non-Small Cell Lung Cancer (NSCLC) is not demonstrated. In present study, miR-489 was down-regulated both in tumor tissues and cells. Inhibition of miR-489 promoted cells invasion by using an invasion assay. Furthermore, miR-489 could regulate SUZ12 as shown by luciferase reporter and Western blot assays. Aberrant miR-489 expression could regulate the molecular changes (E-cadherin, N-cadherin, and Vimentin) of epithelial mesenchymal transition (EMT). In conclusion, our study revealed that miR-489 may play an essential role in the progression of NSCLC.
微小RNA是一类小的非编码RNA，在癌症进展过程中参与多种生物学过程。然而，miR-489在非小细胞肺癌（NSCLC）发生发展中的潜在作用尚未得到证实。在本研究中，miR-489在肿瘤组织和细胞中均呈下调状态。通过侵袭实验发现，抑制miR-489可促进细胞侵袭。此外，荧光素酶报告基因实验和蛋白质免疫印迹实验表明，miR-489可调控SUZ12。miR-489的异常表达可调节上皮-间质转化（EMT）的分子变化（E-钙黏蛋白、N-钙黏蛋白和波形蛋白）。总之，我们的研究表明，miR-489可能在NSCLC的进展中发挥重要作用。
