State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, PR China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, PR China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, PR China.
Acta Biomater. 2018 May;72:381-395. doi: 10.1016/j.actbio.2018.03.051. Epub 2018 Apr 6.
Multipotent human bone marrow mesenchymal stem cells (hBMSCs) are commonly used as seed cells in bone tissue engineering, but their clinical application is limited due to two challenges. One is the expansion of hBMSCs without loss of the stemness, and the other is the stimulation of osteogenic differentiation of hBMSCs when combined with biomaterials. In this study we demonstrated an approach by firstly elucidating the functional effects and optimal concentrations of Si and Sr ions on the proliferation and osteogenic differentiation of hBMSCs, and then designing bioactive bioceramic/alginate hydrogels which could release Si and Sr bioactive ions in the same optimal concentrations range for activation of the cells in vivo. The results showed that Si and Sr ions could synergistically stimulate cell proliferation without losing the stemness. Furthermore, at higher concentrations, Si and Sr ions stimulated osteogenic differentiation instead of enhancing proliferation. The designed bioactive hydrogels revealed activity to stimulate not only the osteogenic differentiation of encapsulated hBMSCs, but also the blood vessel formation in vivo. These results suggested that the design of biomaterials based on the biological function of different material elements was an effective approach for bone tissue engineering applications.
The clinical application of multipotent human bone marrow mesenchymal stem cells (hBMSCs) in bone tissue engineering is limited due to two challenges. One is the expansion of cells without loss of the stemness, and the other is the stimulation of osteogenic differentiation of hBMSCs within the biomaterial scaffolds. Herein, we demonstrated an approach by firstly elucidating the functional effects and optimal concentrations of Si and Sr ions on the proliferation without losing stemness and osteogenic differentiation of hBMSCs, and then designing a bioactive bioceramic/alginate hydrogel which could release Si and Sr ions for in vivo activation of cells. The bioactive hydrogels revealed activity to stimulate not only osteogenic differentiation of encapsulated hBMSCs, but also the blood vessel formation in vivo. Our work provided an effective approach to design effective biomaterials for tissue engineering.
多能人类骨髓间充质干细胞（hBMSCs）通常被用作骨组织工程中的种子细胞，但由于两个挑战，其临床应用受到限制。一个是 hBMSCs 的扩增而不失干性，另一个是与生物材料结合时刺激 hBMSCs 的成骨分化。在这项研究中，我们首先阐明了 Si 和 Sr 离子对 hBMSCs 增殖和成骨分化的功能影响和最佳浓度，然后设计了能够以相同最佳浓度范围释放 Si 和 Sr 生物活性离子的生物活性生物陶瓷/藻酸盐水凝胶，以激活体内细胞。结果表明，Si 和 Sr 离子可以协同刺激细胞增殖而不失干性。此外，在较高浓度下，Si 和 Sr 离子刺激成骨分化而不是增强增殖。设计的生物活性水凝胶显示出不仅刺激包封的 hBMSCs 成骨分化，而且还刺激体内血管形成的活性。这些结果表明，基于不同材料元素的生物学功能设计生物材料是骨组织工程应用的有效方法。
多能人类骨髓间充质干细胞（hBMSCs）在骨组织工程中的临床应用受到两个挑战的限制。一个是细胞的扩增而不失干性，另一个是生物材料支架内 hBMSCs 的成骨分化的刺激。在此，我们首先阐明了 Si 和 Sr 离子对 hBMSCs 增殖而不失干性和成骨分化的功能影响和最佳浓度，然后设计了一种能够释放 Si 和 Sr 离子以在体内激活细胞的生物活性生物陶瓷/藻酸盐水凝胶。生物活性水凝胶显示出不仅刺激包封的 hBMSCs 成骨分化，而且还刺激体内血管形成的活性。我们的工作为设计有效的组织工程生物材料提供了一种有效方法。
