Tiryakioglu Osman, Erkoc Kamuran, Tunerir Bulent, Uysal Onur, Altin H Firat, Gunes Tevfik, Aydin Selim
Department of Cardiovascular Surgery, Bahcesehir University Medical Faculty, Istanbul, Turkey.
Department of Cardiovascular Surgery, Medical Park Bursa Hospital, Bursa, Turkey.
Biomed Res Int. 2015;2015:453748. doi: 10.1155/2015/453748. Epub 2015 Mar 5.
The objective of this study was to examine the effects of iloprost and N-acetylcysteine (NAC) on ischemia-reperfusion (IR) injuries to the gastrocnemius muscle, following the occlusion-reperfusion period in the abdominal aorta of rats.
Forty male Sprague-Dawley rats were randomly divided into four equal groups. Group 1: control group. Group 2 (IR): aorta was occluded. The clamp was removed after 1 hour of ischemia. Blood samples and muscle tissue specimens were collected following a 2-hour reperfusion period. Group 3 (IR + iloprost): during a 1-hour ischemia period, iloprost infusion was initiated from the jugular catheter. During a 2-hour reperfusion period, the iloprost infusion continued. Group 4 (IR + NAC): similar to the iloprost group.
The mean total oxidant status, CK, and LDH levels were highest in Group 2 and lowest in Group 1. The levels of these parameters in Group 3 and Group 4 were lower compared to Group 2 and higher compared to Group 1 (P < 0.05). The histopathological examination showed that Group 3 and Group 4, compared to Group 2, had preserved appearance with respect to hemorrhage, necrosis, loss of nuclei, infiltration, and similar parameters.
Iloprost and NAC are effective against ischemia-reperfusion injury and decrease ischemia-related tissue injury.
本研究的目的是在大鼠腹主动脉阻断再灌注后，研究伊洛前列素和N-乙酰半胱氨酸（NAC）对腓肠肌缺血再灌注（IR）损伤的影响。
40只雄性Sprague-Dawley大鼠随机分为四组，每组数量相等。第1组：对照组。第2组（IR组）：阻断主动脉，缺血1小时后松开血管夹，再灌注2小时后采集血样和肌肉组织标本。第3组（IR + 伊洛前列素组）：在1小时缺血期，经颈静脉导管开始输注伊洛前列素，在2小时再灌注期继续输注。第4组（IR + NAC组）：与伊洛前列素组相似。
第2组的平均总氧化剂状态、肌酸激酶（CK）和乳酸脱氢酶（LDH）水平最高，第1组最低。第3组和第4组这些参数的水平与第2组相比更低，与第1组相比更高（P < 0.05）。组织病理学检查显示，与第2组相比，第3组和第4组在出血、坏死、细胞核丢失、浸润及类似参数方面外观保存较好。
伊洛前列素和NAC对缺血再灌注损伤有效，并可减轻缺血相关组织损伤。
