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姬鼠属(鼠科,啮齿目)林姬鼠和黄胸鼠中两个重复DNA家族的差异

[Divergence of two families of repeated DNA in wood and field mice of the genus Apodemus (Muridae, Rodentia)].

作者信息

Chelomina G N

出版信息

Genetika. 1993 Jul;29(7):1163-71.

PMID:8370512
Abstract

The nDNA of six species of a wood and field mice of the genus Apodemus (2n = 48): peninsulae, sylvaticus, flavicollis, microps, speciosus, agrarius, grey rat Rattus norvegicus as well, and house mouse Mus musculus were studied using EcoRI and HindIII restriction endonucleases with an agarose and polyacrylamide gel electrophoresis and a microdensitometry. It is shown for dispersed sequences that a quarter of 1.85 kb EcoRI genus specific family of repeated DNA is constant but a quarter of 1.35 kb EcoRI repeats, which are specific for more large taxon unit, is variable. The HindIII satDNA of the various species differs in the length of monomer, size of more representative multimer and digestion of the monomers by the same endonuclease of restriction; HindIII satDNA quantity correlates with 1.35 kb EcoRI repeat content. Some nonidentified components are found in Apodemus genomes. The possible ways evaluation of the repetitive DNA are briefly discussed. It was supposed that the evolutionary age of the wood mice is directly proportional to the length of HindIII multimer and inversely proportional to the amount of HIndIII satDNA with a 1.35 kb EcoRI repeats.

摘要

利用EcoRI和HindIII限制性内切酶,通过琼脂糖和聚丙烯酰胺凝胶电泳及微量光密度测定法,对姬鼠属6种林栖和田野小鼠(2n = 48):半岛姬鼠、森林姬鼠、黄颈姬鼠、小嘴姬鼠、中华姬鼠、黑线姬鼠,以及褐家鼠和小家鼠的核DNA进行了研究。结果表明,对于分散序列,1.85 kb EcoRI属特异性重复DNA家族的四分之一是恒定的,但对于更大分类单元特异的1.35 kb EcoRI重复序列的四分之一是可变的。不同物种的HindIII卫星DNA在单体长度、更具代表性的多聚体大小以及同一限制性内切酶对单体的消化方面存在差异;HindIII卫星DNA数量与1.35 kb EcoRI重复序列含量相关。在姬鼠基因组中发现了一些未鉴定的成分。简要讨论了评估重复DNA的可能方法。据推测,林栖小鼠的进化年龄与HindIII多聚体长度成正比,与具有1.35 kb EcoRI重复序列的HIndIII卫星DNA数量成反比。

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