College of Pharmacy, Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang 830011, China.
College of Pharmacy, Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang 830011, China.
J Ethnopharmacol. 2015 Jun 20;168:116-21. doi: 10.1016/j.jep.2015.03.013. Epub 2015 Mar 31.
Ethnopharmacological relevance Apocynum venetum: is widely used in Uygur and traditional Chinese medicine. Modern pharmaceutical studies have shown that leaves of A. venetum have effects of liver protection, antidepressant and regulation of blood pressure. However, it is unclear that which components have pharmacological activities.
The aim was to study chemical constituents of A. venetum and its anti-platelet aggregation activity.
Nephelometery was applied to evaluate anti-platelet aggregation activity of multi-components of A. venetum. Systematic separation components were characterized by HPLC analysis method, and in vitro screening active components by anti-platelet aggregation study.
Ethyl acetate fraction (L-III) and L-III-4 have better anti-platelet aggregation activity than other fractions. The results indicated that isoquercitrin, hyperoside and other flavonoids have anti-platelet aggregation activity in A. venetum.
Our studies provide basis on the endeavors of screening chemicals with that anti-platelet aggregation activity in leaves of A. venetum.
民族药理学相关性 夹竹桃：在维吾尔族和中医中被广泛使用。现代药物研究表明，夹竹桃的叶子具有护肝、抗抑郁和调节血压的作用。然而，目前尚不清楚哪些成分具有药理活性。
本研究旨在研究夹竹桃的化学成分及其抗血小板聚集活性。
采用比浊法评价夹竹桃多组分的抗血小板聚集活性。采用 HPLC 分析方法对系统分离的成分进行表征，并通过抗血小板聚集研究对活性成分进行体外筛选。
乙酸乙酯部分（L-III）和 L-III-4 具有比其他部分更好的抗血小板聚集活性。结果表明，异槲皮苷、金丝桃苷等黄酮类化合物具有抗血小板聚集活性。
本研究为筛选夹竹桃叶中具有抗血小板聚集活性的化学成分提供了依据。
