Camargo Anderson Carlos, Dias Mariane Rezende, Cossi Marcus Vinícius Coutinho, Lanna Frederico Germano Piscitelli Alvarenga, Cavicchioli Valéria Quintana, Vallim Deyse Christina, Pinto Paulo Sérgio de Arruda, Hofer Ernesto, Nero Luís Augusto
1 Departamento de Veterinária, Universidade Federal de Viçosa , Viçosa, Brazil .
Foodborne Pathog Dis. 2015 Apr;12(4):323-6. doi: 10.1089/fpd.2014.1875.
Utensils and equipment from meat-processing facilities are considered relevant cross-contamination points of Listeria monocytogenes to foods, demanding tracking studies to identify their specific origins, and predict proper control. The present study aimed to detect L. monocytogenes in a beef-processing facility, investigating the diversity of serotypes and pulsotypes in order to identify the possible contamination routes. Surface samples from knives (n=26), tables (n=78), and employees hands (n=74) were collected before and during the procedures from a beef-processing facility, in addition to surface samples of end cuts: round (n=32), loin (n=30), and chuck (n=32). All samples were subjected to L. monocytogenes screening according ISO 11.290-1, and the obtained isolates were subjected to serotyping and pulsed-field gel electrophoresis. Listeria spp. were identified in all processing steps, in 61 samples, and L. monocytogenes was detected in 17 samples, not being found only in knives. Eighty-five isolates were identified as L. monocytogenes, from serotypes 1/2c (n=65), 4b (n=13), and 1/2b (n=7), being grouped in 19 pulsotypes. Considering these results, cross-contamination among hands, tables, and beef cuts could be identified. The obtained data indicated the relevance of cross-contamination in the beef-processing facility, and the occurrence of serotypes 1/2b and 4b in beef cuts distributed for retail sale is a public health concern.
肉类加工设施中的器具和设备被认为是单核细胞增生李斯特菌污染食品的相关交叉污染点，需要进行追踪研究以确定其具体来源，并预测适当的控制措施。本研究旨在检测一家牛肉加工设施中的单核细胞增生李斯特菌，调查血清型和脉冲型的多样性，以确定可能的污染途径。除了取自牛腿肉（n = 32）、里脊肉（n = 30）和肩胛肉（n = 32）的终端切块表面样本外，还在一家牛肉加工设施的操作前和操作过程中收集了刀具（n = 26）、工作台（n = 78）和员工手部（n = 74）的表面样本。所有样本均按照ISO 11.290 - 1进行单核细胞增生李斯特菌筛查，对获得的分离株进行血清分型和脉冲场凝胶电泳分析。在所有加工步骤中，共在61个样本中鉴定出李斯特菌属，在17个样本中检测到单核细胞增生李斯特菌，仅在刀具中未检测到。共鉴定出85株单核细胞增生李斯特菌，血清型为1/2c（n = 65）、4b（n = 13）和1/2b（n = 7），分为19个脉冲型。考虑到这些结果，可以确定手部、工作台和牛肉切块之间存在交叉污染。获得的数据表明了牛肉加工设施中交叉污染的相关性，零售销售的牛肉切块中出现血清型1/2b和4b是一个公共卫生问题。
