Departamento de Veterinária, Campus UFV, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil.
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, São Paulo, SP, 05508-000, Brazil.
Probiotics Antimicrob Proteins. 2019 Jun;11(2):696-704. doi: 10.1007/s12602-018-9449-0.
Bacteriocinogenic Enterococcus hirae ST57ACC and Pediococcus pentosaceus ST65ACC strains, previously isolated from artisanal cheese, were evaluated for their safety with the aim to determine whether they could be used as beneficial strains, especially in the control of Listeria monocytogenes. Both isolates survived simulated gastrointestinal conditions and showed high levels of auto- and co-aggregation with L. monocytogenes, although the hydrophobicity of cells varied. Using the agar-spot test with 33 commercial drugs from different groups, only anti-inflammatory drugs and drugs containing loratadine and propranolol hydrochloride were able to affect the growth of the tested strains. Both strains were resistant to 3 out of 11 antibiotics tested by the disc diffusion method, and low frequencies of antibiotic resistance-encoding genes were observed by PCR analysis. Tested strains neither presented biogenic amine-related genes nor produced these substances. Aside from some antibiotic resistance characteristics, the tested strains were considered safe as they lack other virulence-related genes. E. hirae ST57ACC and P. pentosaceus ST65ACC both presented beneficial properties, particularly their ability to survive gastrointestinal conditions and to aggregate with L. monocytogenes, which can facilitate the elimination of this pathogen. Further studies should be conducted to better understand these interactions.
从传统奶酪中分离得到的两株产细菌素屎肠球菌 ST57ACC 和戊糖片球菌 ST65ACC，用于评估其安全性，目的是确定它们是否可以作为有益菌株使用，特别是在控制单核细胞增生李斯特菌方面。这两种分离株都能耐受模拟的胃肠道条件，并与单核细胞增生李斯特菌表现出高水平的自动聚集和共聚作用，尽管细胞的疏水性有所不同。使用琼脂斑点试验，对来自不同组的 33 种商业药物进行检测，只有抗炎药和含有氯雷他定和盐酸普萘洛尔的药物能够影响测试菌株的生长。这两种菌株对 11 种通过纸片扩散法测试的抗生素中的 3 种具有耐药性，通过 PCR 分析观察到低频率的抗生素耐药基因。测试菌株既没有携带生物胺相关基因，也没有产生这些物质。除了一些抗生素耐药特性外，这两种测试菌株被认为是安全的，因为它们缺乏其他与毒力相关的基因。屎肠球菌 ST57ACC 和戊糖片球菌 ST65ACC 都具有有益的特性，特别是它们能够在胃肠道条件下存活并与单核细胞增生李斯特菌聚集，这有助于清除这种病原体。应进一步开展研究以更好地了解这些相互作用。
