Schmidt U, Schmid H, Guder W G
Hoppe Seylers Z Physiol Chem. 1978 Feb;359(2):193-8.
Periportal and perivenous hepatocytes were isolated by microdissection from lyophilized liver slices (16 micrometer) from fed and fasted rats and from a human patient. NADP/NADPH cycling was used to determine fructose-1,6-bisphosphatase activity in the isolated hepatocytes (10 ng dry weight). The periportal hepatocytes contain 3 times as much fructose-1,6-bisphosphatase activity as the perivenous hepatocytes. A 24 h fast led to two-fold increase in the activity in the periportal hepatocytes and a four-fold increase in the perivenous hepatocytes. Fructose-1,6-bisphosphatase parallels closely with the key enzyme phosphoenolpyruvate carboxykinase, and therefore can be considered a suitable marker for gluconeogenic capacity.
通过显微切割从喂食和禁食大鼠以及一名人类患者的冻干肝切片（16微米）中分离出门周和中央静脉周围的肝细胞。利用NADP/NADPH循环来测定分离出的肝细胞（干重10纳克）中的果糖-1,6-二磷酸酶活性。门周肝细胞中的果糖-1,6-二磷酸酶活性是中央静脉周围肝细胞的3倍。禁食24小时导致门周肝细胞中的活性增加两倍，中央静脉周围肝细胞中的活性增加四倍。果糖-1,6-二磷酸酶与关键酶磷酸烯醇式丙酮酸羧激酶密切平行，因此可被视为糖异生能力的合适标志物。
