Department of Regenerative Surgery, Fukushima Medical University, School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan.
Department of Regenerative Surgery, Fukushima Medical University, School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan.
Lung Cancer. 2015 Jun;88(3):310-8. doi: 10.1016/j.lungcan.2015.03.004. Epub 2015 Mar 23.
Metabolic alternations are deemed a hallmark of cancer cells. Among many metabolic pathways, glycolysis and lipogenesis are essential metabolic processes in cancer cells. In this study, we examined the prognostic impact of the combined expression of glycolysis-related glucose transporter 1 (GLUT1) and ATP-citrate lyase (ACLY), which are important molecules in lipogenesis, in patients with non-small cell lung cancer (NSCLC).
GLUT1 and ACLY expression in 134 NSCLC specimens were determined by immunohistochemistry using a tissue microarray (TMA). We examined the overall survival of patients with GLUT1-, ACLY- or GLUT+ACLY-positive expression using Kaplan-Meier analysis. We analyzed the prognostic impact of combined GLUT1 and ACLY expression according to lymph node status using multivariate Cox analysis.
Patients with GLUT1- or ACLY-positive expression exhibited poorer overall survival compared with GLUT1- or ACLY-negative patients. GLUT1-positive/ACLY-positive expression status was associated with the worst overall survival, in contrast with GLUT1 negative/ACLY-negative expression status, which was correlated with the best overall survival (P=0.003). GLUT1-positive/ACLY-positive expression was significantly correlated with poor prognosis in node-negative but not in node-positive patients. Multivariate Cox analysis indicated combined expression of GLUT1 and ACLY was an independent prognostic factor for overall survival in node-negative patients with NSCLC (P=0.049).
These results suggest that the combined expression of GLUT1 and ACLY could be a more valuable prognostic factor than their individual expression in node-negative patients with NSCLC.
代谢改变被认为是癌细胞的一个标志。在许多代谢途径中，糖酵解和脂肪生成是癌细胞的重要代谢过程。在这项研究中，我们研究了与糖酵解相关的葡萄糖转运蛋白 1（GLUT1）和三羧酸循环中的柠檬酸裂解酶（ACLY）的联合表达对非小细胞肺癌（NSCLC）患者的预后影响，这两种蛋白都是脂肪生成中的重要分子。
使用组织微阵列（TMA）通过免疫组织化学检测 134 例 NSCLC 标本中 GLUT1 和 ACLY 的表达。我们使用 Kaplan-Meier 分析检查了 GLUT1、ACLY 或 GLUT+ACLY 阳性表达患者的总生存率。我们根据淋巴结状态使用多变量 Cox 分析分析了联合 GLUT1 和 ACLY 表达的预后影响。
与 GLUT1 或 ACLY 阴性患者相比，GLUT1 或 ACLY 阳性表达的患者总生存率较差。GLUT1 阳性/ACLY 阳性表达状态与最差的总生存率相关，而 GLUT1 阴性/ACLY 阴性表达状态与最佳的总生存率相关（P=0.003）。GLUT1 阳性/ACLY 阳性表达与淋巴结阴性患者的不良预后显著相关，但与淋巴结阳性患者的预后无关。多变量 Cox 分析表明，联合表达 GLUT1 和 ACLY 是非小细胞肺癌淋巴结阴性患者总生存的独立预后因素（P=0.049）。
这些结果表明，与单独表达相比，GLUT1 和 ACLY 的联合表达可能是非小细胞肺癌淋巴结阴性患者更有价值的预后因素。
