Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
Ecotoxicol Environ Saf. 2015 Nov;121:253-7. doi: 10.1016/j.ecoenv.2015.03.015. Epub 2015 Mar 31.
Lipid from microalgae is one of the putative oil resources to facilitate the biodiesel production during this era of energy dissipation and environmental pollution. In this study, the key parameters such as biomass productivity, lipid productivity and lipid content were evaluated at the early stationary phase of Chlamydomonas reinhardtii, CC1010 cultivated in nutrient starved (nitrogen, phosphorous), glucose (0.05%, 0.1%, 0.15% and 0.2%) and vitamin B12 supplementation (0.001%, 0.002% and 0.003%) in Tris-Acetate-Phosphate (TAP) medium. The lipid content in nitrogen starved media was 61% which is 2.34 folds higher than nutrient sufficient TAP medium. Glucose supplementation has lead to proportional increase in biomass productivity with the increasing concentration of glucose whereas vitamin B12 supplementations had not shown any influence in lipid and biomass production. Further, fatty acid methyl ester (FAME) profiling of C. reinhardtii, CC 1010 has revealed more than 80% of total SFA (saturated fatty acid) and MUFA (mono unsaturated fatty acid) content. Quality checking parameters of biodiesel like cetane number, saponification value, iodine number and degree of unsaturation were analyzed and the biodiesel fuel properties were found to be appropriate as per the international standards, EN 14214 and ASTM D6751. Conclusively, among all the treatments, nitrogen starvation with 0.1% glucose supplementation had yielded high lipid content in C. reinhardtii, CC 1010.
微藻油脂是能源消耗和环境污染时代促进生物柴油生产的潜在油源之一。在这项研究中，评估了在营养饥饿（氮、磷）、葡萄糖（0.05%、0.1%、0.15%和 0.2%）和维生素 B12 补充（0.001%、0.002%和 0.003%）条件下，在 TAP 培养基中培养的莱茵衣藻 CC1010 处于早期稳定期的关键参数，如生物量生产力、油脂生产力和油脂含量。在营养缺乏的培养基中，油脂含量为 61%，比营养充足的 TAP 培养基高 2.34 倍。葡萄糖补充导致生物量生产力与葡萄糖浓度成比例增加，而维生素 B12 补充对油脂和生物量生产没有任何影响。此外，对莱茵衣藻 CC1010 的脂肪酸甲酯（FAME）分析表明，总 SFA（饱和脂肪酸）和 MUFA（单不饱和脂肪酸）含量超过 80%。对生物柴油的质量检查参数，如十六烷值、皂化值、碘值和不饱和度进行了分析，发现生物柴油燃料的性质符合国际标准，如 EN 14214 和 ASTM D6751。总之，在所有处理中，氮饥饿与 0.1%葡萄糖补充相结合，使莱茵衣藻 CC1010 的油脂含量最高。
